Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing element or inorganic compound except carbon dioxide
Patent
1995-09-25
1998-12-22
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing element or inorganic compound except carbon dioxide
435 697, 435181, 435188, 435193, 536 232, 536 234, 536 235, 536 2351, 536 2353, C12P 2106, C12P 2104, C12N 910, C07H 2104
Patent
active
058517910
ABSTRACT:
A novel class of fusion proteins based on the ubiquitin-conjugating enzyme, or E2, is described. The fusion proteins include, in addition to the E2 activity, a protein binding ligand having a specific affinity for a target protein. It has been discovered that under cytosolic conditions, such E2 fusions will add a ubiquitin moiety to a target protein. Since ubiquitin addition triggers the endogenous cellular protein degradation pathway, such E2 fusion proteins can be used to selectively target proteins in a host for degradation. Thus, E2 fusion proteins genes can be introduced into transgenic organisms to defeat or inhibit natural activities or traits. The E2 fusion proteins can also be used by introduction into hosts for similar effects.
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Gosink Mark Mattnew
Vierstra Richard David
Patterson Jr. Charles L.
Slobodyansky Elizabeth
Wisconsin Alumni Research Foundation
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