Ubiquitin carrier enzyme E2-F1, purification, production, and us

Details Ubiquitin carrier enzyme E2-F1, purification, production, and us Ubiquitin carrier enzyme E2-F1, purification, production, and us

1993-06-21

1995-01-24

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 74, 4351723, 4352523, 4352402, 4353201, 4351721, 536 232, C12N 910, C12N 1554

Patent

active

053842553

ABSTRACT:
A method for isolating and purifying novel species of E2 ubiquitin-carrier protein, designated E2-F1, is disclosed. A method for preparing enzymatically active fragments of E2-F1 enzyme is also disclosed. The use of purified E2-F1 to produce antibodies is also disclosed. The use of such E2-F1-specific antibodies to detect the presence of E2-F1 in a biological sample, and to inhibit protein degradation are also disclosed. Recombinant DNA molecules which code for E2-F1, and recombinant hosts and vectors which contain E2-F1 coding sequences are also disclosed. The use of such recombinant hosts and vectors to produce E2-F1 protein is also disclosed. The use of purified E2-F1 to identify and to isolate E3 enzyme is also disclosed. Methods for screening substances for the ability to inhibit E2-F1 enzyme activity are also disclosed.

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