Ubiquitin-based split protein sensor

Details Ubiquitin-based split protein sensor Ubiquitin-based split protein sensor

1994-12-07

1996-12-17

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 772, 435 78, 435 79, 435 791, 435 792, 435 793, 435 794, 435 795, 530350, 5303911, 5303913, G01N 3353, C07K 1900

Patent

active

055852459

ABSTRACT:
Disclosed are novel compositions and methods, based on ubiquitin subdomain fusion proteins, which are useful for studying the interactions of two members of a specific binding pair, both of which are predetermined. A preferred embodiment relates to the determination of a predetermined ligand in a sample.

REFERENCES:
patent: 5283173 (1994-02-01), Fields et al.
Cox et al. (1993). Dissecting the structure of a partially folded protein . . . J. Mol. Biol. 234, 483-492.
Guarente (1993). Strategies for the identification of interacting proteins. Proc. Natl. Acad. Sci. USA 90, 1639-1641.
Germino et al. (1993). Screening for in vivo protein-protein interactions. Proc. Natl. Acad. Sci. USA 90, 933-937.
Blackwood and Eisenman (1991). MAX: A helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex with myc. Science 251, 1211-1217.
DeFeo-Jones et al. (1991). Cloning of cDNAs for cellular proteins that bind to the retinoblastoma gene product. Nature 352, 251-254.
Blanar and Rutter (1992). Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos. Science 256, 1014-1018.
Fields and Song (1989). A novel genetic system to detect protein-protein interactions. Nature 340, 245-246.
Chien et al. (1991). The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA 88, 9578-9582.
Stockman et al. (1993). Heteronuclear three-dimensional NMR spectroscopy of a partially denatured protein: The A-state of human ubiquitin. J. Biomolecular NMR 3, 285-296.
S. Fields et al. "The Two-Hybrid System: An Assay for Protein-Protein Interactions", Trends in Genetics 10(8) 286-292 Aug. 1994.

Affiliated with

Also associated with

Rating

Ubiquitin-based split protein sensor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Ubiquitin-based split protein sensor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ubiquitin-based split protein sensor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1990393