Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-02-23
1998-11-10
Walsh, Stephen
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435194, 4353201, 536235, 530350, C12Q 148, C12N 1554, C12N 1563
Patent
active
058342087
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a novel tyrosine kinase and a deoxyribonucleic acid (DNA) coding for the same. More particularly, the present invention is concerned with a novel cytoplasmic tyrosine kinase which is increased with respect to expression amount thereof in accordance with the differentiation of blood cells, and is also concerned with a DNA coding for the same. The tyrosine kinase of the present invention can be advantageously used for screening chemical substances having the capability to inhibit or activate the tyrosine kinase activity of at least the tyrosine kinase of the present invention. Therefore, the present invention is also concerned with a method of screening for such chemical substances. The present invention is also concerned with a replicable recombinant DNA molecule comprising a replicable expression vector and, operably inserted in the vector, a DNA coding for the tyrosine kinase of the present invention; a microorganism or animal cell transformed with the replicable recombinant DNA molecule; an antibody reactive with a polypeptide comprising as an immunogen at least part of an amino acid sequence of the tyrosine kinase of the present invention; a sense DNA prepared from the cDNA coding for the tyrosine kinase of the present invention, and an anti-sense DNA which is complementary to the sense DNA; and a sense RNA prepared from the cDNA coding for the tyrosine kinase of the present invention and an anti-sense RNA which is complementary to the sense RNA.
2. Discussion Of Related Art
Human blood contains various types of blood cells, and each of them plays a physiologically important role. For example, erythrocytes carry oxygen in a human body, platelets block bleeding, and leukocytes form an immune system to protect a human body against is infection. These various types of blood cells are derived from hematopoietic stem cells in bone marrow.
Recent studies show that hematopoietic stem cells undergo differentiation into various types of blood cells, osteoclasts, mast cells and the like, when stimulated by various hematopoietic-stimulating factors or various environmental factors. However, the mechanism of the differentiation of hematopoietic stem cells has not yet been fully elucidated. Recent studies also show that a tyrosine kinase greatly participates in the development and differentiation of bodies of animals and insects. It has been considered that a tyrosine kinase does also greatly participate in the differentiation of hematopoietic stem cells. For example, it has been reported that c-kit, which is one of receptor tyrosine kinases, is expressed on the surfaces of hematopoietic stem cells, and functions as a receptor for a hematopoietic growth factor and a mast cell growth factor (see Witte, Cell 63: 5, 1990). This receptor tyrosine kinase controls the differentiation of hematopoietic stem cells.
Tyrosine kinases are enzymes which phosphorylate tyrosine residues of a protein, and the physiologically active sites thereof each consist of about 250 amino acid residues. Tyrosine kinases each have a plurality of well conserved amino acid sequences (see Hanks et al., Science 241: 42, 1988). A tyrosine kinase gene fragment may be obtained by preparing a DNA corresponding to the conserved amino acid sequence and conducting a reverse transcription-polymerase chain reaction (RT-PCR) using the prepared DNA as a primer (see Wilks, Methods in Enzymology 200: 533, 1991).
SUMMARY OF THE INVENTION
It is known that a tyrosine kinase plays an important role in control of transcription and in signal transmission, and that a mutation of the gene coding for a tyrosine kinase, or a viral infection may cause malignant alteration of cells.
Tyrosine kinases are classified into two classes, i.e., those of the receptor type and those of the cytoplasmic type. A cytoplasmic tyrosine kinase has not yet been elucidated as compared to a receptor tyrosine kinase, so that it has been strongly desired to make clear the characteristics of the cytoplasm
REFERENCES:
patent: 5618829 (1997-04-01), Takayangi et al.
Bennett, et al. J. Biol. Chem. vol. 269. No. 2. pp. 1068-1074 1994.
Accession No.:T06085 ESTTD03974 Homo Sapiens cDNA clone HFBD023 Situation to e-Sre Kinase. Adams et al. Nature Genetics vol. 4 pp. 256-267 (1993).
MeVicar et al. Oncogene vol. 9, pp. 2037-2044 (1994).
Sakano et al. Oncogene vol. 9. pp. 1155-1161 (1994).
"The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains" Steven K. Hanks, et al., reported in Science, vol. 241, Jul. 1, 1988, pp. 42-51 (English reference).
"Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction" by Andrew F. Wilks, reported in Proc. Natl. Acad. Sci., vol. 86, Mar. 1989, pp. 1603-1607 (English reference).
"Cloning of a complementary DNA for a protein for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60.sup.c-src ", reported in Nature, vol. 351, May 2, 1991, pp. 69-72 (English reference). Nada et al.
Asahi Kasei Kogyo Kabushiki Kaisha
Basham Daryl A.
Walsh Stephen
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