Type II restriction endonuclease SgrAI

Details Type II restriction endonuclease SgrAI Type II restriction endonuclease SgrAI

1990-11-16

1992-07-28

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435199, 435897, C12P 1934, C12N 922

Patent

active

051340690

ABSTRACT:
The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Streptomyces. It can be used to recognize and cleave the double-stranded DNA sequence ##STR2## and its complementary sequence.

REFERENCES:
Tautz, N., et al. (1990), Nuc. Acids Res. 18(10), 3087.
Laue, F., et al. (1990) Nuc. Acids Res. 18(11), 3421.
Tautz, N., et al, (1990), Nuc. Acid Res. 18(16), 4991.

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