Type II restriction endonuclease, Pme I, obtainable from Pseudom

Details Type II restriction endonuclease, Pme I, obtainable from Pseudom Type II restriction endonuclease, Pme I, obtainable from Pseudom

1991-06-03

1993-03-23

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435874, C12N 922

Patent

active

051963302

ABSTRACT:
The present invention provides a novel type II restriction endonuclease obtainable from Pseudomonas mendocina. The endonuclease known as Pme I, recognizes the following nucleotide sequence and has a cleavage point indicated by the arrows:

REFERENCES:
Endow, et al. J. Mol. Biol. 112:521 (1977).
Waalwijk, et al. Nucleic Acids Res. 5:3231 (1978).
Gingeras, et al., Proc. Natl. Acad. Sci. U.S.A. 80:402 (1983).
Lunnen, et al. Gene 74:25-32 (1988).
Sanger, F. et al., Proc. Natl. Acad. Sci. U.S.A. 74:5463-5467 (1977).
Brown, N. L. et al., J. Mol. Biol. 140:143-148 (1980).

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