Type II restriction endonuclease obtainable from Pseudomonas alc

Details Type II restriction endonuclease obtainable from Pseudomonas alc Type II restriction endonuclease obtainable from Pseudomonas alc

1990-05-10

1992-03-24

Patterson, Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 91, C12N 922, C12P 1934

Patent

active

050988395

ABSTRACT:
The present invention provides a novel Type II restriction endonuclease obtainable from Pseudomonas alcaligenes. The endonuclease known as PacI, recognizes the following nucleotide sequence and has a cleavage point indicated by the arrows: ##STR1## Also described is a process for obtaining PacI from P. alcaligenes.

REFERENCES:
Roberts, R. J. (1989), Nucl. Acids Res. 17 (suppl.), r347-r389.
Endov et al., J. Mol. Biol., 112:521 (1977).
Waalwijk et al., Nucleic Acids Res., 5:3231 (1978).
Gingeras et al., Proc. Natl. Sci. Acad., U.S.A., 80:402 (1983).
Lunnen et al., Gene 74:25-32 (1988).

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