Type II restriction endonuclease, CstMI, obtainable from...

Details Type II restriction endonuclease, CstMI, obtainable from... Type II restriction endonuclease, CstMI, obtainable from...

2007-03-06

2007-03-06

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S320100, C435S252300, C536S023200

Reexamination Certificate

active

10616689

ABSTRACT:
In accordance with the present invention, there is provided a novel type II restriction endonuclease, obtainable fromCorynebacterium striatumM82B, hereinafter referred to as “CstMI”, which endonuclease:(1) recognizes the nucleotide sequence 5′-AAGGAG-3′ in a double-stranded DNA molecule as shown below,5′-AAGGAGN20↓-3′3′-TTCCTCN18↑-5′(wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and N represents either G, C, A, or T);(2) cleaves said sequence at the phosphodiester bonds between the 20th and the 21th nucleotides 3′ to the recognition sequence in the 5′-AAGGAG-3 strand of the DNA, and between the 18th and 19th nucleotides 5′ to the recognition sequence in the complement stand, 5′-CTCCTT-3′, to produce a 2 base 3′extension; and(3) possesses a second enzymatic activity that recognizes the same DNA sequence, 5′-AAGGAG-3′, but modifies this sequence by the addition of a methyl group to prevent cleavage by the CstMI endonuclease activity.

REFERENCES:
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