Type II restriction endonuclease BtgZI, obtainable from...

Details Type II restriction endonuclease BtgZI, obtainable from... Type II restriction endonuclease BtgZI, obtainable from...

2006-04-18

2006-04-18

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

Reexamination Certificate

active

07029900

ABSTRACT:
In accordance with the present invention, there is provided a novel restriction endonuclease obtainable fromBacillus thermoglucosidasius36A (NEB#1384), hereinafter referred to as “BtgZI”, which endonuclease:(1) recognizes the nucleotide sequence 5′-GCGATG-3′ in a double-stranded DNA molecule as shown below,5′-GCGATGNNNNNNNNNN↓-3′(SEQ ID NO:9)3′-CGCTACNNNNNNNNNNNNNN↑-5′ (wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and N represents either G, C, A, or T);(2) cleaves said sequence in the phosphodiester bonds between the 10th and the 11th nucleotides 3′ to the recognition sequence in the 5′-GCGATG-3 strand of the DNA, and between the 14th and 15th nucleotides 5′ to the recognition sequence in the complement stand, 5′-CATCGC-3′, to produce a 4 base 5′ extension; and(3) cleaves double-stranded pBR322 DNA to produce 3 fragments of 2892, 1181 and 288 base pairs.

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