Type II restriction endonucleae MCRI

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435199, C12N 922, C12P 1934

Patent

active

051340682

ABSTRACT:
Type II restriction endonuclease McrI is disclosed. This endonuclease has recognition sequence: ##STR1## wherein R is G or A and Y is C or T, and the cleavage site indicated by the arrows. The endonuclease is preferably obtained from organism of genus Micrococcus. It can be used to recognize and cleave double stranded DNA sequence:

REFERENCES:
Kessler, C., et al. (1985) Gene 33, 13.
Brensing-Kuppers, et al. (1990) FEBS Letts 264(2), 218-222.

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