Type II group B Streptococci polysaccharide

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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260112B, 424 85, 424 88, 435101, 536 1, 536 18, A61K 3173, C08B 3700

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043248870

ABSTRACT:
Culturing Streptococcus agalactiae in a nutrient broth containing at least 10 g/l of glucose while maintaining the pH at 6-8 by the addition of alkaline material produces polysaccharide antigen including a fraction having a molecular size from 8-11.times.10.sup.5 d. By employing a broth free from constituents having a molecular size above 10.sup.5, the antigens can be separated from the broth in high yield after harvesting the organisms. Vaccination of humans with Streptococcus agalactiae polysaccharide antigens having molecular sizes above 5.times.10.sup.5 produces in selected individuals specific antibody levels above 200 .mu.g/ml of antisera; globulins fractionated from such antisera contain most of the antibody and can be used for passive immunization.

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