Two step process for coating of antibodies to a solid phase

Details Two step process for coating of antibodies to a solid phase Two step process for coating of antibodies to a solid phase

1992-06-26

1995-03-21

Saunders, David

Chemistry: analytical and immunological testing

Thyroid hormone tests

435 792, 436518, 436531, G01N 33543, G01N 33545

Patent

active

053995007

ABSTRACT:
A process for coating an antibody on a substrate includes two steps. A secondary antibody to a primary antibody is coated on the substrate followed by a coating with primary antibody in the presence of blocking and stabilizer agents.

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Kapyaho, P. et al., Scand. J. Clin. Lab. Lab. Invest. 49; Effect of complement binding on a solid-phase immunometric TSH assay (1989), pp. 211-216.
Sarma, J. et al., Life Sciences, vol. 38, Enzyme Linked Immuno sorbent Assay (ELISA) for B-Endorphin and Its Antibodies, pp. 1723-1732.
Oshima, M. et al., Immunological Investigatons, 18(7), Comparison of Peptide-Coating Conditions in Solid Phase Plate Assays for Detection of Anti-Peptide Antibodies, (1989) 841-851.
Sankolli, G. M. et al., Journal of Immunological Methos, 104, Improvement in the antibody binding characteristics of microtitre wells by pretreatment with anti-IgG Fc immunoglobulin, (1987) pp. 191-194.
Lovborg, U., Guide to Solid Phase Immunoassays.

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