Two step lateral flow assay methods and devices

Details Two step lateral flow assay methods and devices Two step lateral flow assay methods and devices

2011-01-18

2011-01-18

Nguyen, Bao-Thuy L (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S004000, C435S005000, C435S287100, C435S287700, C435S970000, C435S975000, C436S514000, C436S518000, C436S507000, C436S530000, C436S807000, C436S821000, C436S810000, C422S051000, C422S051000, C422S051000

Reexamination Certificate

active

07871781

ABSTRACT:
Lateral flow assay devices and methods for detecting an analyte in a sample which comprises a plurality of nonspecific binding pair members are adapted for two step determinations. In one embodiment, a method for identifying IgE antibodies in a sample comprises applying a sample to a sample port of a device, wherein the device is adapted to deliver the sample to a lateral flow matrix having a plurality of IgE antigen species immobilized at respective positions at a first location, allowing the sample to travel along the lateral flow matrix through the immobilized plurality of IgE antigen species to a second location downstream of the first location and allowing sample to dissolve a water-soluble mark, applying liquid buffer to the lateral flow matrix to mobilize labeled reagent which is adapted to bind anti-IgE antibody and which is dried on the lateral flow matrix at a location upstream of the sample delivery location, and allowing labeled reagent to bind any IgE antibody bound to the immobilized IgE antigen species therein.

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