Two-step enzyme method for preparing 7-aminocephalosporanic...

Details Two-step enzyme method for preparing 7-aminocephalosporanic... Two-step enzyme method for preparing 7-aminocephalosporanic...

2011-08-23

2011-08-23

Pak, Yong (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S004000, C435S119000, C435S025000, C435S069100, C435S071100, C435S252300, C435S320100, C435S193000, C536S023200

Reexamination Certificate

active

08003358

ABSTRACT:
The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeastTrigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, β-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.

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Molla et al. “Overexpression inEscherichia coliof a Recombinant ChimericRhodotorula gracilisD-Amino Acid Oxidase” Protein Expression and Purification 14, (1998) pp. 289-294.
Ishiye et al. “Nucleotide sequence and expression inEscherichia coliof theCephalosporin acylasegene of aPseudomonas strain” Biochimica et Biophysicia Acta, 1132 (1992) pp. 233-239.

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