Two-step calibration method for dry chemical analysis element

Chemistry: analytical and immunological testing – Automated chemical analysis – Condition or time responsive

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436 43, 436 46, 428 63, 428 67, G01N 3700

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057862230

ABSTRACT:
The concentration of an analyte in a sample liquid is determined on the basis of the optical density of a chemical analysis element spotted with the sample liquid according to a standard curve representing the relation between the concentration and the optical density of the analysis element which is obtained when the concentration is measured using an analysis element of a first lot. A plurality of first standard liquids containing the analyte in known levels are spotted onto analysis elements of a second lot, the concentrations of the analyte in the first standard liquids are determined according to the standard curve and a first calibration curve for correcting the concentrations of the analyte is prepared. A plurality of second standard liquids containing the analyte in known levels are spotted onto analysis elements of the second lot, the concentrations of the analyte in the second standard liquids are determined according to the standard curve on the particular analysis system, the concentrations of the analyte in the second standard liquids thus determined are corrected according to the first calibration curve, and a second calibration curve for correcting the concentrations of the specific analyte in the second standard liquids thus corrected is prepared.

REFERENCES:
patent: 4043756 (1977-08-01), Sommervold
patent: 4884213 (1989-11-01), Iwata et al.
patent: 5424212 (1995-06-01), Pinsl-Ober et al.
patent: 5550053 (1996-08-01), Salpeter
patent: 5583049 (1996-12-01), Altman et al.

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