Two-site immunoassays using monoclonal antibodies of different c

Details Two-site immunoassays using monoclonal antibodies of different c Two-site immunoassays using monoclonal antibodies of different c

1983-02-16

1984-10-02

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving iga, igd, ige, or igm

436519, 436548, 436804, 436808, 436819, 436828, 436824, G01N 3354, G01N 3356

Patent

active

044748922

ABSTRACT:
Two-site immunometric assays for multideterminant antigens are described in which the antigen is reacted with an immobilized monoclonal antibody directed against one antigen determinant and a second monoclonal antibody that is directed against a distinct antigenic determinant and is of a different class or subclass than the immobilized monoclonal antibody. The second monoclonal antibody is labeled in direct versions of the assay and is reacted with a labeled antibody against it in indirect versions of the assay. The immobilizing medium and classes (subclasses) of the antibodies may be selected so as to reduce the likelihood of nonspecific binding enhance sensitivity and/or permit signal amplification.

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