Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is inorganic
Patent
1997-06-20
2000-07-11
Housel, James C.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is inorganic
435 71, 435 75, 435 792, 435 795, 435174, 435181, 435971, 436507, 436512, 436513, 436518, 436523, 436524, 436525, 436532, 436538, 436547, 436548, 436811, 436821, 436824, G01N 33553
Patent
active
060871881
ABSTRACT:
A method of detecting an antibody in a sample using a labelling compound and comprising the steps of mixing a ligand antigen, antibody or hapten bound to biotin with the sample; an antibody directed against the antibody to be detected bound to paramagnetic particles; and a chemiluminescent acridinium compound bound to avidin or streptavidin to form a solid phase complex; separating the solid phase from the liquid phase; and analyzing the separated solid phase for the presence of chemiluminescent complex.
REFERENCES:
patent: 4163779 (1979-08-01), Harte et al.
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4444879 (1984-04-01), Foster et al.
patent: 4496654 (1985-01-01), Katz et al.
patent: 4535057 (1985-08-01), Dreesman et al.
patent: 4745181 (1988-05-01), Law et al.
patent: 4935339 (1990-06-01), Zahradnik
patent: 4946958 (1990-08-01), Campbell et al.
patent: 5028524 (1991-07-01), Fujisawa et al.
patent: 5210017 (1993-05-01), Carlsson et al.
patent: 5395938 (1995-03-01), Ramakrishman.
"Magnetic Solid-Phase Enzyme Immunoassay for the Quantitation of Antigens and Antibodies: Application to Human Immunoglobulin E"; Methods In Enzymology; vol. 73; 1981; pp. 471-482.
"Avidin-Biotin Coupling as a General Method for Preparing Enzyme-Based Fiber-Optic Sensors"; Analytical Chemistry; May 15, 1989; vol. 61, No. 10; pp. 1069-1072.
"Improved Immunoglobulin M Serodiagnosis in Lyme Boreoliosis by Using a .mu.-Capture Enzyme-Linked Immunosorbent Assay with Biotinylated Borrelia burgdorferi Flagella"; Journal of Clinical Microbiology; Jan.1991; vol. 29, No. 1; pp. 166-173.
"The Use of Avidin-Biotin Interaction in Immunoenzymatic Techniques"; The Journal of Histochemistry and Cytochemistry; vol. 27, No. 8; pp. 1131-1139; 1979.
"Avidin-Biotin System in Enzyme Immunoassays"; Methods in Enzymology; 1990, vol. 184; pp. 469-481.
"Biotin-labelled antigen screening test for toxoplasma IgM antibody"; J. Clin. Pathol.; 1989; 42:206-209.
"Capture assay for specific IgE An improved quantitative method"; Journal of Immumological Methods; 157(1993) 65-72.
"Measurement of antigen-specific mouse IgE by a fluorometric reverse (IgE-capture) ELISA"; Journal of Immunological Methods; 116 (1989) 181-187.
Product Brochure; Magic.RTM. Lite Sq.TM..
"Rapid Detection of Antigen-specific Mouse IgE by Enzyme-linked Immunosorbent Assay (ELISA)"; RJ Strouse et al.; J Immunoassay 1991;12(1):113-24.
"An Enzyme-linked Immunosorbent Assay for Cow's Milk Protein-specific IgE Using Biotinylated Antigen. Avoidance of Interference by Specific IgG"; A. Plebani et al.; J Immunol Methods 1986 Jun. 24;90(2):241-6.
"The Use of Acridinium Ester-labelled Streptavidin in Immunoassays"; RC Hart et al.; J Immunol Methods 1987 Jul. 16:101(1):91-6.
"Effect of Surfactants on the Intensity of Chemiluminescence Emission from Acridinium ester labelled proteins"; FJ Bagazgoitia et al.; J Biolumin Chemilumin 1988 Jul.-Sep.;2(3):121-8.
Methods in Enzymology, 184(1990) Everly Conway de Macario et al: "Slide Immunoenzymatic Assay for Human Immunoglobin E", p. 501-p. 507.
Methods in Enzymology, 184(1990) C.J. Strasburger et al: "Two-Site and Competitive Chemiluminescent Immunoassays", p. 481-p. 496.
Jacobsen L., Evaluation of the Magic Lite SQ Specific IgE Assay, Clinical Chemistry, vol. 36, No. 6, p. 1189, 1990.
Bousquet, J., Comparison between RAST and Pharmacia CAP system: A new automated specific IgE assay, J. Allergy Clinical Immunology, vol. 85, No. 6, pp. 1039-1042, 1990.
Kleine-Tebbe, J. Comparison between Magic Lite and CAP system: two automated specific IgE antibody assays, Clinial and Experimental Allergy, vol. 22, pp. 475-484, 1992.
Wilchek, M., The Avidin-Biotin Complex in Bioanalytical Aplications, Analytical Biochemistry, vol. 171, pp. 1-32, 1988.
Wilchek, M., The Avidin-Biotin Complex in Immunology, Immunology Today, vol. 5, No. 2, pp. 39-43, 1984.
Ipsen Hans-Henrik
Johansen Niels
ALK A/S
Housel James C.
Nguyen Bao-Thuy L.
LandOfFree
Two-site immunoassay for an antibody with chemiluminescent label does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Two-site immunoassay for an antibody with chemiluminescent label, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Two-site immunoassay for an antibody with chemiluminescent label will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-541417