Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-12-30
1998-03-24
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
34 80, 34 82, 34472, 34477, 435 79, 435 794, 435963, 436518, 436531, 436808, 436826, G01N 33573
Patent
active
057311579
ABSTRACT:
An allergen immunoassay method features the use of a combination of a) closely controlled 1) elevated temperatures for assay reactions, 2) low temperatures for reagents and samples, 3) times for assay steps and especially assay reaction times, 4) reagent concentrations, and 5) reagent amounts; b) the use of a fast and accurate method of sample preparation that removes dust and contaminants; c) the stabilization of samples to avoid auto- and antibody degradation and unwanted effects of sample contaminants; and d) the formation of a colored product to determine the amount of a specific allergen. This combination provides an assay that can be completed in a few hours while retaining the precision, accuracy, sensitivity and response curve of previous methods requiring much longer periods of time. The assay is especially suitable for computer control using a robotic liquid distribution system and allows for the determination of four different specific allergens in one hundred sixty samples in duplicate with standards and controls in an eight hour period with a significant reduction in the number of steps and attended technician time over previous assays.
REFERENCES:
patent: 3593498 (1971-07-01), Semon
patent: 3902971 (1975-09-01), Fletcher et al.
patent: 4251923 (1981-02-01), Kuri
patent: 4256697 (1981-03-01), Baldwin
patent: 4358424 (1982-11-01), Weber et al.
patent: 4453955 (1984-06-01), Cullen et al.
patent: 4545958 (1985-10-01), Dopatka
patent: 4560647 (1985-12-01), Stocker
patent: 4661128 (1987-04-01), Bachhofer et al.
patent: 4670405 (1987-06-01), Stetter et al.
patent: 4690890 (1987-09-01), Loor et al.
patent: 4777021 (1988-10-01), Wertz
patent: 4780285 (1988-10-01), Kuypers et al.
patent: 4822732 (1989-04-01), Sandstrom et al.
patent: 4851357 (1989-07-01), Yamashina
patent: 4931402 (1990-06-01), Abplanalp
patent: 5038852 (1991-08-01), Johnson et al.
patent: 5063090 (1991-11-01), Wannlund
patent: 5073346 (1991-12-01), Partanen et al.
patent: 5110551 (1992-05-01), Michal
patent: 5149634 (1992-09-01), Bradley
patent: 5183740 (1993-02-01), Ligler et al.
patent: 5187084 (1993-02-01), Hallsby
patent: 5266272 (1993-11-01), Griner et al.
Cole-Parmer Instrument company Product Catalog 1991/1992, p. 219.
Frank et al., "Automation of DNA Sequencing Reactions and Related Techniques: A Workstation for Micromanipulations of Liquids"; Biotechnology, vol. 6, pp. 1211-1213 (1988).
Godfrey, T. et al., "Comparison of Key Characteristics of Industrial Enzymes by Type and Source." in: Godfrey, Industrial Enzymology (England, MacMillan Ltd., 1983), pp. 466-502.
Dambmann, C., et al. "How Enzymes Got Into Detergents." Developments in Industrial Microbiologly, vol. 12, (1971), pp. 11-23.
Dambmann, C. et al. "The Variety of Serine Proteases and Their Industrial Significance." in: Turk et al., Proteinases and Their Inhibitors (New York, Permagon Press, 1981), pp. 231-244.
Flindt, M. H. L., "Pulmonary Disease Due to Inhalation of Derivatives of Bacillus subtilis Containing Proteolytic Enzyme." The Lancet, (1969), pp. 1177-1181.
Pepys, J. et al., "Allergic Reactions of the Lungs to Enzymes of Bacillus subtillis." The Lancet, (1969), pp. 1181-1184.
Gilson, J. C. et al., "Biological Effects of Proteolytic Enzyme Detergents." Thorax, vol. 31, (1976), pp. 621-631.
Dunn, E. et al., "The Use of NN'-Dimethylcasein in the Determination of Proteolytic Enzymes in Washing Products and Airborne Dust Samples." Analyst vol. 96, (1971), pp. 159-163.
Rothgeb, T. M. et al., "The Raw Material, Finished Products, and Dust Pad Analysis of Detergent Proteases Using a Small Synthetic Substrate." Journal Americal Oil Chemists' Society, vol. 65, (1988), pp. 806-810.
Miller, L. S., et al., "Inhibition Enzyme Immunoassay for the Detection of Airborne Detergent Enzymes Causing Occupational Allergy." Developments in Industrial Microbiology, vol. 31 (1990), pp. 213-219.
Lowry O. H., et al., "Protein Measurement with the Folin Phenol Reagent." Journal of Biological Chemistry, vol. 193, (1951) pp. 265-275.
Peters. T., "Serum Albumin." in: Anfinsen, B. et al., Advances in Protein Chemistry, vol. 37, (New York, Academic Press, 1985), pp. 161-245.
Claeyssens, M., "The Use of Chromophoric Substrates and Specific Assays in the Study of Structure-Activity Relationships of Cellulolytic Enzymes." in: Aubert, J. P. et al., Biochemistry and Genetics of Cellulose Degradation (New York, Academic Press, 1988), pp. 393-397.
American Conference of Governmental Industrial Hygienists (ACGIH). Documentation of Threshold Limit Values. Fourth edition, Cincinnati, Ohio, (1980) pp. 374-375.
Agarwal, M. K. et al. "Immunochemical Quantitation of an Airborne Proteolytic Enzyme, Esperase.RTM., In a Consumer Products Factory." American Industrial Hygeiene Association Journal, vol. 47. No. 2 (1986), pp. 138-143.
Swanson, M. C. et al. "Immunochemical Quantification and Particle Size Distribution of Airborne Papain in a Meat Portioning Facility." American Industrial Hygiene Association Journal, vol. 53, No. 1 (1992), pp. 1-5.
Voller, A. et al., "Enzyme Immunoassays with Special Reference to ELISA Techniques." Journal of Clinical Pathology, vol. 31 (1978), pp. 507-520.
Wells, J. D. et al., "Detection of Airborne Industrial Papain by a Radioimmunoassay." American Industrial Hygiene Association Journal, vol. 42, Apr. (1981), pp.321-322.
Chien, P. T. "The Development of a Fluorometric Method for the Assay of Subtilisins." American Industrial Hygiene Association Journal, vol. 39, Oct. (1979), pp. 808-816.
Fulwiler, R. D. et al., "Evaluation of Detergent Enzymes in Air." American Industrial Hygiene Association Journal, Apr. (1972), pp. 201-211.
Hendricks, M. H. "Measurement of Enzyme Laundry Product Dust Levels and Characterisitcs in Consumer Use." Journal of the American Oil Chemists' Society, Jun. (1970), pp. 207-211.
Paixao, L. M. et al., "Automated Bioassay of Proteolytic Enzymes in Detergents" Journal of the American Oil Chemists' Society, Oct. (1969), pp. 511-514.
Markland, F. S. et al., "Subtilisins: Primary Structure, Chemical and Physical Properties" in: Boyer, P. D., The Enzymes, vol. 3, (New York Academic Press, 1971), pp. 561-608.
Waller, M. "The Hydrolysis of Human IgG with Subtilisin" American Journal of Clinical Pathology, vol. 31, (1975), pp. 358-364.
Data Index 5, "Current Industrial Enzyme Assays and Unit Definititions" in: Godfrey and Reichelt, Industrial Enzymology, (England, MacMillan Ltd., 1983), pp. 553-557.
Agarwal, M. K., et al., "An Immunochemical Method to Measure Atmospheric Allergens" Journal Allergy and Clinical Immunology, vol. 68, No. 3, (1981), pp. 194-200.
Feder, J., et al. "Stabilization of Proteolytic Enzymes in Solution." Biotechnology and Bioengineering, vol. XX, (1978), pp. 1865-1872.
Friedman, S., et al. "Enzymic Activity of Proteases in Detergent Systems: Comparison of Assay Methods and the Role of Interfering Substances." Journal of the American Oil Chemists' Society, vol. 46, (1968), pp. 81-84.
Swisher, R. D., "Detergent Enzymes: Biodegradation and Environmental Acceptability." Bioscience, vol. 19, (1969), pp. 1093-1094.
Miller, L. S., et al. "Enzyme Imunoassay for the Quantitation of an Alkaline Protease in Airborne Samples." U. S. Enviromental Protection Agency Res. Dev., (Rep.), EPA/600/D-89/189, EPA Date (1988).
Bhullar Balwant S.
Miller Larry S.
Moore Victor S.
Tuttle Richard S.
Hutzell Paula K.
Pollick Philip J.
The Procter and Gamble Company
Wolski Susan C.
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