Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1996-10-11
1998-06-02
Leary, Louise
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 721, 435968, 436 63, 436800, 356 401, 356 39, 250200, 2504591, 2503381, C12Q 100, G01N 33567, G01N 3353
Patent
active
057597677
ABSTRACT:
A method of measuring an analyte present in animal (e.g., human) tissue or fluids such as blood or plasma. The analyte is multi-photon excitable (e.g., two-photon excitable) at a first wavelength at which the animal tissue is substantially non-absorbing. The analyte fluoresces at a second wavelength upon being excited at the first wavelength. The animal tissue is irradiated with radiation at the first wavelength so as to excite the analyte through absorption by the analyte of two or more photons of the radiation at the first wavelength. Excitation of the analyte results in a fluorescent emission from the analyte of radiation at the second wavelength. The emission at the second wavelength is detected, and the concentration of analyte determined based on the detected emission.
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Burke Thomas G.
Gryczynski Ignacy
Lakowicz Joseph R.
Malak Henryk
Lakowicz Joseph R.
Leary Louise
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