Two-phase optical assay with unitized container and double...

Details Two-phase optical assay with unitized container and double... Two-phase optical assay with unitized container and double...

2006-07-21

2010-12-14

Yu, Melanie (Department: 1641)

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

C435S007100, C435S007920, C436S523000

Reexamination Certificate

active

07851229

ABSTRACT:
Improved single-container, two-phase optical assays for analytes are provided which are faster and require less steps than conventional two phase optical assays. The assays of the invention involve first mixing and incubating an assay mixture including a buffer, solid particles (e.g., agarose beads), an analyte-containing sample, and an affinity agent operable to bind analyte(s) to the solid particles, followed by separation of the mixture into a particle-rich phase and a substantially particle-free phase. In one aspect, the settling step is gravity-induced and is instrumentally monitored to determine when substantially full separation has occurred. Thereafter, the respective phases may be photometrically measured to obtain qualitative and/or quantitative information about the analyte(s). It has been found that measurements taken with only one sensor set before and after settling of the particle-rich fraction give scientifically valid results as a two phase optical assay.

REFERENCES:
patent: 4409335 (1983-10-01), Hanamoto et al.
patent: 5369037 (1994-11-01), Hansen
patent: 5674699 (1997-10-01), Saunders et al.
patent: 6294342 (2001-09-01), Rohr et al.

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