Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1996-09-26
1998-07-07
Grimes, Eric
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352523, 43525411, 4353201, 435325, 536 232, 536 2431, C12N 510, C12N 1557, C12N 1563, C12P 2102
Patent
active
057767599
ABSTRACT:
The present invention provides two novel human cathepsin proteins (HCPs) and polynucleotides encoding HCPs. The invention provides for genetically engineered expression vectors and host cells comprising the nucleic acid sequence encoding HCPs. The invention also provides for the production and use of antibodies to HCPs in pharmaceutical compositions for the treatment of disease processes that include cancers, inflammation, metastasis and peptide and proenzyme processing. In addition, the invention provides for the production and use of inhibitors of HSPs in pharmaceutical compositions for the treatment of diseases. The invention also describes diagnostic assays which utilize the polynucleotide to hybridize with the transcripts encoding HCPs. The invention also provides for the use of antisense molecules in pharmaceutical compositions as a therapeutics in cancers, inflammation, metastasis and peptide and proenzyme processing.
REFERENCES:
patent: 5501969 (1996-03-01), Hastings et al.
Wiederanders et al., J. Biol. Chem. 267:13703-13713, 1992.
Horst et al., Biochem. J. 273:355-361, 1991.
Joseph et al., J. Clin. Invest. 81: 1621-1729, 1988.
Adams et al., Nature Genetics 4:373-380, 1993.
Mori, K. et al., "Molecular Cloning of a Novel Mouse Aspartic Protease-like Protein That is Expressed Abundantly in the Kidney", FEBS Letters, vol. 401, pp. 218-222, Jan. 20, 1997.
Baldwin, E.T., et al., "Crystal structures of native and inhibited forms of human cathespin D: Implications for lysosomal targeting and drug design" Proc. Natl. Acad. Sci., 90:6796-6800 (1993).
Becker, M.M., et al., "Cloning and Characterization of the Schistosoma japonicum Aspartic Proteinase Involved in Hemoglobin Degradation" J. Biol. Chem., 270:24496-24501 (1995).
Cataldo, A.M., et al., "Gene Expression and Cellular Content of Cathepsin D in Alzheimer's Disease Brain: Evidence of Early Up-Regulation of the Endosomal-Lysosomal System" Neuron, 14:671-680 (1995).
Cavilles, V., "Cathespin D gene is controlled by a mixed promoter, and estrogens stimulate only TATA-dependent transcription in breast cancer cells" Proc. Natl. Acad. Sci., 90:203-207 (1993).
Consonni, R., et al., "Further characterization of platelet-aggregating cysteine proteinase activity in thrombotic thrombocytopenic purpura" BR. J. Haematol., 87:321-324 (1994).
Dickinson, A.J., et al., "An Immunohistochemical and Prognostic Evaluation of Cathespin D Expression in 105 Bladder Carcinomas" J. Urol., 154:237-241 (1995).
Diedrich, J.F., et al., "Neuropathological Changes in Scrapie and Alzheimer's Disease Are Associated with Increased Expression of Apolipoprotein E and Cathepsin D in Astrocytes" J. Virol., 65:4759-4768 (1991).
Evin, G., et al., "Candidate .lambda.-Secretases in the Generation of the Carboxyl Terminus of the Alzheimer's Disease .beta.A4 Amyloid: Possible Involvement of Cathepsin D" Biochemistry, 334:14185-14192 (1995).
Faust, P.L., et al., "Cloning and sequence analysis of cDNA for human cathepsin D" Proc. Natl. Acad. Sci., 82:4910-4914 (1985).
Huisman, W., et al., "Role of Individual Cathepsins in Lysosomal Protein Digestion as Tested by Specific Inhibitors" Biochim. Biophys. Acta, 370:297-307 (1974).
Keyszer, G.M., et al., "Comparative Analysis of Cathepsin L, Cathepsin D, and Collagenase Messenger RNA Expression in Synovial Tissues of Patients with Rheomatoid Arthritis and Osteoarthritis, by in situ Hybridization" Arthritis & Rheum., 38:976-984 (1995).
Kopitz J., et al., "Protein catabolism in fibroblasts cultured from patients with mucolipidosis II and other lysosomal disorders" Biochem. J., 295:577-580 (1993).
Liaudet, E., et al., "Transfected Cathepsin D Stimulates High Density Cancer Cell Growth by Inactivating Secreted Growth Inhibitors" Cell Growth & Differ., 6:1045-1052 (1995).
Long, B.J., et al., "Reduced levels of cathespin D associated with tamoxifen resistance and estrogen independent in the ZR-75-1 human breast cancer cell line" Cancer Letters, 99:233-238 (1996).
Mantel, D., et al., "Comparison of cathepsin protease activities in brain tissue from normal cases and cases Alzheimer's disease, Lewy body dementia, Parkinson's disease and Huntington's disease" J. Neurol. Sci., 131:65-70 (1995).
McKerrow, J.H. et al., "The Proteases and Pathogenicity of Parasitic Protozoa" Annu. Rev. Microbiol., 47:821-53 (1993).
Mizouchi, T., et al., "Both cathepsin B and cathepsin D are necessary for processing of ovalbumin as well as for degradation of class II MHC invariant chain" Immunol. Letters, 43:189-193 (1994).
Ritchlin, C., et al., "Sustained and Distinctive Patterns of Gene Activation in Synovial Fibroblasts and Whole Synovial Tissue Obtained from Inflammatory Synovitis" Scand. J. Immunol., 40:292-298 (1994).
Rochefort, H., "Biological and Clinical Significance of Cathepsin D in Breast Cancer" Acta Oncol., 31:125-130 (1992).
Rosenthal, P.J., et al., "A Malarial Cysteine Proteinase is Necessary for Hemoglobin Degradation by Plasmodium falciparium" J. Clin. Invest., 82:1560-1566 (1988).
Ross, J.S., et al., "Quantitative Immunohistochemical Determination of Cathepsin D Levels in Prostatic Carcinoma Biopsies" Am. J. Clinc. Pathol., 104:36-41 (1995).
Saftig, P., et al., "Mice deficient for the lysosomal proteinase cathepsin D exhibit progressive atrophy of the intestinal mucosa and profound destruction of lymphoid cells" EMBO J., 14:3599-3608 (1995).
Smith, A.M., et al., "Adult Schistosoma mansoni express cathepsin L proteinase activity" Mol. Biochem. Parasitol., 67:11-19 (1994).
Troen, B.R., et al., "Downstream Sequences Mediate Induction of the Mouse Cathepsin L Promoter by Phorbol Esters" Cell Growth & Differ., 2:23-31 (1991).
Bandman Olga
Coleman Roger
Billings Lucy J.
Grimes Eric
Incyte Pharmaceuticals Inc.
LandOfFree
Two novel human cathepsin proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Two novel human cathepsin proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Two novel human cathepsin proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1204620