Optics: measuring and testing – By shade or color – With color transmitting filter
Patent
1986-04-11
1989-02-07
McGraw, Vincent P.
Optics: measuring and testing
By shade or color
With color transmitting filter
2504581, 356318, 356246, G01N 2164, G01N 2105
Patent
active
048027685
ABSTRACT:
A reference system utilizes pulsed arc light excitation which excitation pulses are directed onto a flow cell containing a fluorescent dye. Fluorescent light emitted from the dye is guided to a photomultiplier tube which converts it to electrical pulses. A portion of each excitation light pulse is guided by a light pipe onto a PIN diode light detector which converts these light signals to electrical pulses. A LED reference light source is pulsed to generate a plurality of reference light pulses one of which occurs between each excitation pulse. A portion of each of these pulses is guided to each of the two light detectors and two more series of electrical pulses are generated. A microprocessor then reads the four electrical pulses resulting from each pair of light pulses and performs a computation on the resulting numbers which indicates the relative concentration of the target concentration being assayed. An optical system masks the excitation light pulses and the emitted light pulses to minimize the amount of scattered excitation light that gets into the emitted light optical channel and spatially integrates the images of the arc yielding the excitation light to control the stability of the image projected onto the photomultiplier tube. The light pipe and an output lens spatially integrates the image of the excitation light mask and focuses this light on the PIN diode so that the dancing image of the arc does not modulate with the variations of the PIN diode cathode and destabilize its output signal.
REFERENCES:
patent: 3746452 (1973-07-01), Teboul et al.
patent: 3891853 (1975-06-01), Kremen et al.
patent: 3918812 (1975-11-01), Holm
patent: 4074939 (1978-02-01), Rabl
patent: 4133873 (1979-01-01), Noller
patent: 4320970 (1982-03-01), Dowben et al.
patent: 4516856 (1985-05-01), Popelka
patent: 4622468 (1986-11-01), Stefanski et al.
Lambert et al., Canadian Journal of Microbiology, 21:8:1232-1235, (1975) "Etude de la Stabilite et de la Degradation de la Fluorescence dans la Technique d'Immunofluorescence (FTA-ABS) a l'Aide de Lasers a Impulsions".
Kasatiya et al., Applied Microbiology, 27:5:838-843, (1974), "Use of Tunable Pulsed Dye Laser for Quantitative Fluorescence in Syphilis Serology (FTA-ABS Test)".
Bergquist et al., Scandinavian Journal of Immunology, 2:1:37-44, (1973), "The Pulsed Dye Laser as a Light Source for the Fluorescent Antibody Technique".
Bigliardi Achille M.
Gifford Charles R.
Fish Ron
McGraw Vincent P.
Sclavo Inc.
LandOfFree
Two light source reference system for a fluorometer does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Two light source reference system for a fluorometer, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Two light source reference system for a fluorometer will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1081749