Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-03-03
1999-11-09
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 794, 435 21, 435 25, 435 28, 435962, 435968, 435970, 435973, 435 6, 436524, 436535, 436809, G01N 3353, G01N 33551
Patent
active
059812028
ABSTRACT:
A method for assaying a substance is provided in which an optical image is obtained from spots of light emitted from a chemiluminescent material capable of undergoing a luminescent reaction. The method comprises: (a) a step of distributing solid particles on the surface of a support, the solid particles having a diameter of not more than 100 .mu.m and on its surface carrying an antibody being able to catch a catalyst previously bound to the second antibody (or its analogue, such as Fab') through binding an antigen, and the support being permeable to a liquid and capable of absorbing light emitted from chemiluminescent material; (b) a step of immersing the support into a solution containing the chemiluminescent material to bring the catalyst over the support into contact with the chemiluminescent material to cause luminescent reactions including fluorescence; (c) a step of two-dimensionally picking up an optical image including an image formed by luminescence and fluorescence, and converting it into electric signals; (d) a step of storing the measured two-dimensional image data of the optical image in a image processing device; and (e) a step of manipulating the stored signals to form a new image in the same processing device.
REFERENCES:
patent: 3690837 (1972-09-01), Witz et al.
patent: 4652533 (1987-03-01), Jolley
patent: 4948975 (1990-08-01), Erwin et al.
patent: 5089424 (1992-02-01), Khalil et al.
patent: 5096807 (1992-03-01), Leaback
patent: 5106732 (1992-04-01), Kondo et al.
patent: 5108704 (1992-04-01), Bowers et al.
patent: 5133866 (1992-07-01), Kauvar
patent: 5275951 (1994-01-01), Chow et al.
patent: 5320944 (1994-06-01), Okada et al.
patent: 5415839 (1995-05-01), Zaun et al.
patent: 5508200 (1996-04-01), Tiffany et al.
patent: 5512451 (1996-04-01), Kricka
Akhavan-Tafti et al., "CCD Camera Imaging for the Chemiluminescent Detecting of Enzymes Using New Ultrasensitive Reagents," J. Biolumin. Chemilumin 9(3): 155-164, 1994.
Hooper et al., "Low-Light Imaging Technology in the Life Sciences," J. Biolumin. Chemilumin. 9(3): 113-122, 1994.
Patent Abstracts of Japan, Publication No. 020.51063 Publication Date: Feb. 21, 1990 "Detection of Specific Antigen Or Fine Object Having The Same.".
Maly et al, "A Single-Photon Imaging System for the Simultaneous Quantitation of Luminescent Emissions from Multiple Samples", Analytical Biochemistry, vol. 168, 1988, pp. 462-469.
Motsenbocker et al, "Improvements to Enhanced Horseradish Peroxidase Detection Sensitivity", J. Biolumin Chemilumin 1994, vol. 9, pp. 15-20.
Ugelstad et al, "Magnetic Polymer Particles fo Cell Separation", Antibody-Mediated Methods, pp. 74-77 Bone Marrow Transplantation, V2, S2, 1987.
Ishikawa et al, "Enzyme-Labeling of Antibodiew and Their Fragments for Enzyme Immunoassay and Immunohistochemical Staining", Journal of Immunoassay, 4(3), 1983, pp. 209-327.
Biosensor Laboratories Co., Ltd.
Chin Christopher L.
Do Pensee T.
LandOfFree
Two-dimensional solid phase assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Two-dimensional solid phase assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Two-dimensional solid phase assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1454038