Two-component system that controls bacterial membrane synthesis

Details Two-component system that controls bacterial membrane synthesis Two-component system that controls bacterial membrane synthesis

2007-03-13

2007-03-13

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S320100, C435S252330, C536S024100

Reexamination Certificate

active

10288986

ABSTRACT:
The present invention discloses a mutantNeisseriahaving extensive membrane blebbing, both an indicium and a cause of virulence in the gonococcus and meningococcus. Methods are disclosed for making and characterizing the mutant, bmrRS. Methods are disclosed for isolating bmrRS membranes for use as a vaccine. Methods are also disclosed for the use of the mutant for determining the virulence of clinical samples ofN. gonorrhoeaeandN. meningitidis. Methods are also disclosed for the screening of antibiotics targeted to virulentNeisseria.

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Galanos, C..,et al. ,“A New Method for the Extraction of R Lipopolysaccharides”,Eur. J. Biochem., (9), (1969),245-249.
Lee, Na-Gyong.,et al. ,“Mutation of the htrB Locus ofHaemophilus influenzaeNontypable Strain 2019 Is Associated with Modifications of Lipid A and Phosphorylation of the Lipo-oligosaccharide”,J. Biol. Chem., 270(45), (1995),27151-27159.
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Pettit, Robin.K. ,et al. ,“Characterization of naturally elaborated blebs from serum-susceptible and serum-resistant strains ofNeisseria gonorrhoese”, Mol. Microbiol., 6, (1992),723-728.
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Watnick, Randolph.S. ,et al. ,“Escherichia coliNusA is required for efficient RNA binding by phase HK022 Nun protein”,PNAS, 95, (Feb. 1998),1546-1551.
Wren, Brendan.W. ,et al. ,“Degenerate PCR Primers for the Amplification of Fragments from Genes Encoding Response Regulators from a Range of Pathogenic Bacteria”,FEMS Microbiol. Letters, 99, (1992),287-291.
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