Two component stain composition for producing a Giemsa blood sta

Details Two component stain composition for producing a Giemsa blood sta Two component stain composition for producing a Giemsa blood sta

1984-03-28

1986-06-17

Wallen, Thomas

Compositions

Nonreactive analytical, testing, or indicating compositions

424 2, 424 3, 436 8, 436 17, 436 18, G01N 3100, G01N 100, B66D 304

Patent

active

045955247

ABSTRACT:
Disclosed is a novel, 2 component Giemsa stain. The stain comprises a first component which is an aqueous solution of methylene blue, Azure B and a preservative and a second component which is an acetone/dimethyl formamide/water solvent system containing the sodium salt of eosin Y and a buffer. The Giemsa stain effect is obtained by first fixing a blood slide with methanol and then applying the freshly mixed first and second components of the stain. The slide is then rinsed and dried to prepare it for examination.

REFERENCES:
patent: 2992971 (1961-07-01), Millman et al.
patent: 3546334 (1970-12-01), Lerner et al.
patent: 3876375 (1975-04-01), Maurukas
patent: 4299726 (1981-11-01), Crews et al.
patent: 4302355 (1981-11-01), Turner et al.
patent: 4382075 (1983-05-01), Liqo et al.
patent: 4392864 (1983-07-01), Helfrich et al.
Lillie, Histopathologic Technique and Practical Histochemistry, 3rd ed., McGraw-Hill, New York (1965), pp. 584-589.
Lillie, et al., Stain Technology, 54 (1979), pp. 47-48.

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