Twin-arginine translocation in Bacillus

Details Twin-arginine translocation in Bacillus Twin-arginine translocation in Bacillus

2011-03-01

2011-03-01

Burkhart, Michael (Department: 1633)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S069100, C435S069700, C435S070100, C435S071100, C435S320100, C435S252100

Reexamination Certificate

active

07897742

ABSTRACT:
Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as aBacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is anEscherichia colior a member of the genusPantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3850752 (1974-11-01), Schuurs et al.
patent: 3939350 (1976-02-01), Kronick et al.
patent: 3996345 (1976-12-01), Ullman et al.
patent: 4275149 (1981-06-01), Litman et al.
patent: 4277437 (1981-07-01), Maggio
patent: 4366241 (1982-12-01), Tom et al.
patent: 4816567 (1989-03-01), Cabilly et al.
patent: 5641671 (1997-06-01), Bos et al.
patent: 5804409 (1998-09-01), Bos et al.
patent: 7214773 (2007-05-01), Kolkman
patent: 0 444 759 (1991-09-01), None
patent: WO 99/51753 (1999-10-01), None
patent: WO 02/055717 (2002-07-01), None
Hirose et al., “Proteome analysis ofBacillus subtilisextracellular proteins: a two-dimensional protein electrophoretic study,” Microbiology, vol. 146, pp. 65-75, 2000.
Holubova et al., “Transfer of Liposome-Encapsulated Plasmid DNA toBacillus subtilisProtoplasts and Calcium-TreatedEscherichia coliCells,” Folia Microbiol. vol. 30, pp. 97-100, 1985.
Hulett et al., “Evidence for Two Structural Genes for Alkaline Phosphatase inBacillus subtilis,” J. Bacteriol. vol. 172, pp. 735-740, 1990.
Hynds et al., “The Sec-independent Twin-arginine Translocation System Can Transport Both Tightly Folded and Malfolded Proteins across the Thylakoid Membrane,” J. Biol. Chem. vol. 273, pp. 34868-3474, 1998.
Jack et al., “Constitutive Expression ofEscherichia coli tatGenes Indicates an Important Role for the Twin-Arginine Translocase during Aerobic and Anaerobic Growth,” J. Bacteriology, vol. 183, pp. 1801-1804, 2001.
Jongbloed, Jan D. H. et al., << TatC Is a Specificity Determinant for Protein Secretion via the Twin-arginine Translocation Pathway, >>Journal of Biological Chemistry, vol. 275, No. 52, pp. 41350-41357, Dec. 29, 2000.
Keifer et al., “Negative charged amino acid residues play an active role in orienting the Sec-independent Pf3 coat protein in theEscherichia coliinner membrane,” EMBO, vol. 16, pp. 2197-2204, 1997.
Klenk et al., “The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeonArchaeoglobus fulgidus,” Nature, vol. 394, pp. 364-370, 1998.
Kroll et al., “A Multifunctional Prokaryotic Protein Expression System: Overproduction, Affinity Purification, and Selective Detection,”DNA and Cell Biology, vol. 12, No. 5, pp. 441-453, 1993.
Kunst et al., “The complete genome sequence of the Gram-positive bacteriumBacillus subtilis,” Nature, vol. 390, pp. 249-264, Nov. 20, 1997.
Kyte et al., “A Simple Method for Displaying the Hydropathic Character of a Protein,” J. Mol. Biol. vol. 157, pp. 105-132, 1982.
Laemmli U. K. “Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4,” Nature, vol. 227, pp. 680-685, 1970.
Maddox et al., “Elevated Serum Levels in Human Pregnancy of a Molecule Immunochemically Similar to Eosinophil Granule Major Basic Protein,” J. Exp. Med., vol. 158, pp. 1211-1226, Oct. 1983.
Tjalsma, Harold et al., “Signal Peptide-Dependent Protein Transport inBacillus subtilis: a Genome-Based Survey of the Secretome,”Microbiology and Molecular Biology, vol. 64, No. 3, pp. 515-547, Sep. 2000.
Towbin et al., “Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications,” Proc. Natl. Acad. Sci. USA, vol. 76, pp. 4350-4354, 1979.
Vagner et al., “A vector for systematic gene inactivation inBacillus subtilis,” Microbiology, vol. 144, pp. 3097-3104, 1998.
Van Dijl et al., “Non-functional expression ofEscherichia colisignal peptidase I inBacillus subtilis,” J. Gen. Microbiology, vol. 137, pp. 2073-2083, 1991.
Van Dijl et al., “Signal peptidase I overproduction results in increased efficiencies of export and maturation of hybrid secretory proteins inEscherichia coli,” Mol. Gen. Genet, vol. 227, pp. 40-48, 1991.
Van Dijl et al., “Identification of the Potential Active Site of the Signal Peptidase SipS ofBacillus subtilis,” J. Biol. Chem., vol. 270, pp. 3611-3618, 1995.
Van Dijl., Jan Maarten et al., << Functional genomic analysis of theBacillus subtilisTat Pathway for protein secretion, >>Journal of Biotechnology, pp. 243-254, Sep. 25, 2002.
Vorobjeva et al., “Transformation ofBacillus megateriumProtoplasts by Plasmid DNA,” FEMS Microbiology Letters 7, pp. 261-263, 1980.
Weiner et al., “A Novel and Ubiquitous system for Membrane Targeting and Secretion of Cofactor-Containing Proteins,” Cell, vol. 93, pp. 93-101, 1998.
Weinrauch et al., “Plasmid Marker Rescue Transformation Proceeds by Breakage-Reunion inBacillus subtilis,” Journal of Bacteriology, vol. 169, No. 3, pp. 1205-1211, Mar. 1987.
Weinrauch et al., “Plasmid Marker Rescue Transformation inBacillus subtilis,” Journal of Bacteriology, vol. 154, No. 3, pp. 1077-1087, Jun. 1983.
Wu et al., “Bacterial Twin-Arginine Signal Peptide-Dependent Protein Translocation Pathway: Evolution and Mechanism,” J. Mol. Microbiol. Biotechnol. vol. 2, pp. 179-189, 2000.
Yu et al., “The Cytochrome bc Complex (menaquinone: Cytochrome c Reductase) inBacillus subtilisHas a Nontraditional Subunit Organization,” J. Bacteriol. vol. 177, pp. 6751-6760, 1995.
Altschul el et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database programs,” Nucl. Acids Res., vol. 25, pp. 3389-3402, 1997.
Antelmann el al., “Expression of a Stress—and Starvation-induced dps/pexB—Homologous Gene Is Controlled by the Alternative Sigma Factor oBinBacillus Bacillus,” J. of Bacteriology, vol. 179, pp. 7251-7256, 1997.
Antelmann el al., “Phosphate Starvation-Inducible Proteins ofBacillus Bacillus: Proteomics and Transcriptional Analysis,” J. of Bacteriology, vol. 182, pp. 4478-4490, 2000.
Bakhiet et al., “Studies on Transfection and Transformation of Protoplasts ofBacillus larvae, Bacillus subtilis,andBacillus popilliae,” Applied and Environmental Microbiology, vol. 49, No. 3, pp. 577-581, Mar. 1985.
Benton et al., “Steering λgt Recombinant Clones by Hybridization to Single Plaques in situ,”Science, vol. 196, No. 4286, pp. 180-182, Apr. 8, 1977.
Berger and Kimmel, Guide to Molecular Cloning Techniques, Methods in Enzymology, vol. 152, Academic Press, San Diego, CA (1987).
Berks B., “A common export pathway for proteins binding complex redox cofactors,” Mol Microbiol. vol. 22, pp. 393-404, 1996.
Berks et al., “The Tat protein export pathway,” Mol Microbiol. vol. 35, pp. 260-274, 2000.
Bernhardt et al., “Specific and general stress proteins inBacillus subtilis—a two-dimensional protein electrophoresis study,” Microbiol. vol. 143, pp. 999-1017, 1997.
Database EMBL, Blattner F.R. et al., << Hypothetical 11.3 KD Protein in UDP-RFAH Intergenic Region (TATA Protein), Database accession No. 065938, XP002133194, 1998.
Blum et al., “Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels,” Electrophoresis, vol. 8, pp. 93-99, 1987.
Bogsch et al., “Pathway specificity for a ΔpH-dependent precursor thylakoid lumen protein is governed by a ‘Sec-avo

Affiliated with

Also associated with

Rating

Twin-arginine translocation in Bacillus does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Twin-arginine translocation in Bacillus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Twin-arginine translocation in Bacillus will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2631889