Twin-arginine translocation in Bacillus

Details Twin-arginine translocation in Bacillus Twin-arginine translocation in Bacillus

2011-02-08

2011-02-08

Burkhart, Michael (Department: 1633)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C435S183000, C424S192100

Reexamination Certificate

active

07884192

ABSTRACT:
Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as aBacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is anEscherichia colior a member of the genusPantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

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