Tumour necrosis factor binding ligands

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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5303881, 435335, 4241451, C07K 1624

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active

059590874

ABSTRACT:
The present invention relates to ligands which bind to human tumour necrosis factor alpha (TNF) in a manner such that upon binding of these ligands to TNF the biological activity of TNF is modified. In preferred forms the ligand binds to TNF in a manner such that the induction of endothelial procoagulant activity of the TNF is inhibited; the binding of TNF to receptors on endothelial cells is inhibited; the induction of fibrin deposition in the tumour and tumour regression activities of the TNF are enhanced; and the cytotoxicity and receptor binding activities of the TNF are unaffected or enhanced on tumour cells. The ligand is preferably an antibody, F(ab) fragment, single domain antibody (dABs) single chain antibody or a serum binding protein. It is preferred, however, that the ligand is a monoclonal antibody or F(ab) fragment thereof.

REFERENCES:
Watanabe et al., Gan To Kagaku Ryoho, Aug. 1986, 13(8) p2625-9.
Neda et al., Sapporo Med J 56 (2):305-318., 1987.
Tracey et al., Nature, 1987, 330:662.
Natanson et al., Ann. Int. Med., 1994, 120(9):771.
Bringman et al., Hybridoma, 6(5):489, 1987.

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