Tryptophan overproducer mutants of cereal crops

Plant husbandry – Process

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A01H 106

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active

045818479

ABSTRACT:
This invention is directed to the production of novel plant genotypes or lines which produce increased free tryptophan levels and are resistant to inhibition by analogs which normally inhibit the activity of the enzyme anthranilate synthase before alteration. This invention further relates to genes encoding such enzymes, and to processes for utilizing these novel genes and enzymes. Further products of the invention include plants, plant tissues and seeds which exhibit resistance to such analogs and overproduce tryptophan resulting from expression of genes encoding analog resistant anthranilate synthase enzyme.

REFERENCES:
patent: 4443971 (1984-04-01), Chaleff
Processed Plant Protein Foodstuffs, Altschul, 1958, Acad. Press, Inc., New York, pp. 878-880, 883, 884, and 891 cited.
Amino Acids, Nutrition and Stress, Miflin et al., pp. 391-414, Genetic Eng. of Plants, 1983, Plenum Press, N.Y.
Selection of Plant Cell Lines, Widholm, pp. 99-113, Plant Tissue Culture as a Source of Biochemicals, 1980, CRC Press.
Handbook of Plant Cell Culture, Evans et al., 1983, Macmillan Publ. Co., N.Y., pp. 43-45, 50, 401.
Widholm, Biochim. Biophys, Acta, 261: 44-51, 52-58, (1972).
Widholm, Biochim. Biophys. Acta, 279: 48-57 (1972).
Singh and Widholm, Biochem. Gen. 13: 357-367 (1975).
Carlson and Widholm, Physiol. Plant 44: 251-255 (1978).
Ranch et al., Plant Physiol. 71: 136-140 (1983).
Chaleff and Ray, Science, 233: 1148-1153, (1984).

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