Truncated recombinant major outer membrane protein antigen...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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Details

C435S007200, C435S007220, C435S007320, C435S007900, C435S007920, C435S007940, C435S007950, C530S350000

Reexamination Certificate

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07846675

ABSTRACT:
A recombinant, refolded non-fusion polypeptide expressed from a truncated r56 gene of the causative agent of scrub typhus,Orientia tsutsugamushifor the Karp, Kato and Gilliam strains has been produced. The invention is useful for detecting prior exposure to scrub typhus, screening for and/or identification of at least one infectious strain-similarity (i.e. a Karp-like, Kato-like or Gilliam-like strain) based on its strength of reaction toward a truncated protein and as a component in vaccine formulation sand production of immune globulins for passive prophylaxis and immunity in subjects.

REFERENCES:
patent: 5439808 (1995-08-01), Blake et al.
patent: WO99/34215 (1999-07-01), None
Stover et al. The 56-kilodalton major protein antigen of Rickettsia tsutsugamushi: molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope; 1990. 58(7): 2076-2084.
Kim et al. High-level expression of a 56-kilodalton protein gene (bor56) of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays. 1993. 31(3):598-605.
Ohashi et al. Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi. Infect Immuno. 57(5): 1427-1431.
Ohashi et al. Cloning and sequencing of the gene (tsg56) encoding a type-specific antigen from Rickettsia tsutsugamushi. Gene. 1990. 91(1):119-120.

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