Truncated recombinant major outer membrane protein antigen...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...

Reexamination Certificate

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C424S234100, C424S184100, C530S300000, C530S350000, C530S825000, C514S002600

Reexamination Certificate

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07638130

ABSTRACT:
A recombinant, refolded non-fusion polypeptide expressed from a truncated r56 gene of the causative agent of scrub typhus,Orientia tsutsugamushifor the Karp, Kato and Gilliam strains has been produced. The invention is useful for detecting prior exposure to scrub typhus, screening for and/or identification of at least one infectious strain-similarity (i.e. a Karp-like, Kato-like or Gilliam-like strain) based on its strength of reaction toward a truncated protein and as a component in vaccine formulation sand production of immune globulins for passive prophylaxis and immunity in subjects.

REFERENCES:
patent: 5439808 (1995-08-01), Blake et al.
patent: WO99/34215 (1999-07-01), None
Ohashi et al (Infect.Immun., 1989, 57(5): 1427-1431).
Oshahi et al (Gene. 1990. 91: 119-122).
Stover et al. The 56-kilodalton major protein antigen ofRickettsia tsutsugamushi: molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope; 1990. 58(7): 2076-2084.
Kim et al. High-level expression of a 56-kilodalton protein gene (bor56) ofRickettsia tsutsugamushiBoryong and its application to enzyme-linked immunosorbent assays. 1993. 31(3):598-605.
Ohashi et al. Purification and partial characterization of a type-specific antigen ofRickettsia tsutsugamushi. infect immuno 57(5): 1427-1431.
Ohashi et al. Cloning and sequencing of the gene (tsg56) encoding a type-specific antigen fromRickettsia tsutsugamushi. Gene. 1990. 91(1):119-120.

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