Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Patent
1993-10-13
1995-05-30
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
43525233, 4353261, C12N 902, C12N 1572
Patent
active
054200288
ABSTRACT:
A catalytically active, truncated human cholesterol 7.alpha.-hydroxylase (CYP7) is provided. The truncated human CYP7 can be made recombinantly and thus recovered in relatively large amounts. Vectors and host cells for recombinant expression are provided, as well as an antibody which specifically recognizes an epitope of catalytically active, truncated human CYP7. Also provided is a method for screening a compound for its effect on expression of non-truncated human CYP7, and a method for screening a compound for its effect on non-truncated human CYP7 enzyme activity.
REFERENCES:
Karam, W. G. et al., "Polymorphisms of Human Cholesterol 7.alpha.-Hydroxylase", Biochem. and Biophys. Res. Comm. 185(2):588-595 (1992).
Breslow, J. L. et al., "Transgenic Mouse Models of Lipoprotein Metabolism and Atherosclerosis", Proc. Natl. Acad. Sci. USA 90: 8314-8318 (1993).
Cohen, J. C. et al., "Cloning of the Human Cholesterol 7.alpha.-Hydroxylase Gene (CYP7) and Localization to Chromosome 8q11-q12", Genomics 14: 153-161 (1992).
Nishimoto, M. et al., "Structure of the Gene Encoding Human Liver Cholesterol 7.alpha.-Hydroxylase", Biochimica. et Biophysica. Acta. 1172: 147-150 (1992).
Thompson, J. F. et al., "Cholesterol 7.alpha.-Hydroxylase Promoter Separated from Cyclophilin Pseudogene By Alu Sequence", Biochimica et Biophysica Acta 1168: 239-242 (1993).
Li, Y. C. et al., "The Expression of a Catalytically Active Cholesterol 7.alpha.-Hydroxylase Cytochrome P450 in Escherichia coli", The Journal of Biological Chemistry 266(29): 19186-19191 (1991).
Molowa, D. T. et al., "Transcriptional Regulation of the Human Cholesterol 7.alpha.-Hydroxylase Gene", Biochemistry 31: 2539-2544 (1992).
Nishimoto, M. et al., "Structural Analysis of the Gene Encoding Rat Cholesterol .alpha.-Hydroxylase, The Key Enzyme for Bile . . . ", The Journal of Biological Chemistry 266(10): 6467-6471 (1991).
Jelinek, D. F. et al., "Structure of the Rat Gene Encoding Cholesterol 7.alpha.-Hydroxylase", Biochemistry 29(34): 7781-7785 (1990).
Chiang, J. Y. L. et al., "Cloning and 5'-Flanking Sequence of a Rat Cholesterol 7.alpha.-Hydroxylase", Biochimica et Biophysica Acta 1132: 337-339 (1992).
Lusis, Aldons J., "The Mouse Model for Atherosclerosis", TCM 3(4): 135-143 (1993).
Dueland, Svein et al., "Effect of Dietary Cholesterol and Taurocholate on Cholesterol 7.alpha.-hydroxylase and Hepatic LDL Receptors in Inbred Mice", Journal of Lipd Research 34: 923-931 (1993).
Dueland, Svein et al., "Expression of 7.alpha.-Hydroxylase in Non-hepatic Cell Results in Liver Phenotypic Resistance of the Low Density Lipoprotein Receptor to Cholesterol Repression", Journal of Biological Chemistry 267(32): 22695-22698 (1992).
Noshiro et al. (1990) Febs Lett 268(1), 137-140; Abstract only.
Sambrook et al in "Molecular Cloning: A Laboratory Manual" 2nd Ed.; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 1989, 16.1-16.72 and 17.1-17.41.
Ramirez, M. I., et al, (1994) Mol. Cell. Biol. 14(4), 2809-2821.
Northeastern Ohio Universities
Patterson Jr. Charles L.
LandOfFree
Truncated human cholesterol 7.alpha.-hydroxylase, method of prod does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Truncated human cholesterol 7.alpha.-hydroxylase, method of prod, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Truncated human cholesterol 7.alpha.-hydroxylase, method of prod will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-360862