Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2006-05-02
2006-05-02
Achutamurthy, Ponnathapu (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S440000, C435S025000, C435S252300, C435S320100, C435S069100, C536S023200, C536S023700
Reexamination Certificate
active
07037696
ABSTRACT:
A truncated glucanase with an improved thermal stability and a higher specific enzymatic activity than the wild-type enzyme. The truncated glucanase is obtained by removing a number of amino acid residues from the C-terminal of the wild-type 1,3-1,4-β-D-glucanase ofFibrobacter succinogenes. The removal of the C-terminal amino acid residues can be conducted at the genetic level by modifying the gene encoding for the wild type enzyme using, for example, a PCR-based method. Or, it can also be conducted at the protein level by first producing the wild-type enzyme protein and then subjecting the wild-type protein to certain protease action to remove a portion of its C-terminal.
REFERENCES:
patent: 6103511 (2000-08-01), Li et al.
Teather et al., DNA Sequence of a Fibrobacter succinogenes Mixed-Linkage -Glucanase (1,3-1,4- -D-Glucan 4-Glucanohydrolase) Gene,Journal of Bacteriology,pp. 3837-3841 (1990).
Irvin et al., “Cloning and Expression of a Bacteroides succinogenes Mixed-Linked -Glucanase (1,3-1,4- -D-Glucan 4-Glucanohydrolase) Gene inEscherichia coli”, Applied and Environmental Microbiology,pp. 2672-2676 (1988).
Erfle et al., “Purifcation and properties of a 1,3-1,4- -D-glucanase (lichenase, 1,3-1,4- -D-glucan 4-glucanohydrolase, EC 3.2.1.73) from Bacteroides succinogenes cloned inEscherichia coli”,Biochem J.pp. 833-841 (1988).
Chen Jui-Lin
Shyur Lie-Fen
Yang Ning-Sun
Academia Sinica
Achutamurthy Ponnathapu
Cohen & Pontani, Lieberman & Pavane
Pak Yong D.
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