Truncated Cry35 proteins

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S023710, C424S093200

Reexamination Certificate

active

07985892

ABSTRACT:
This invention provides truncated Cry35 proteins that surprisingly and unexpectedly have increased pesticidal activity as compared to full-length Cry35 proteins. The subject invention also includes polynucleotides that encode these truncated proteins, transgenic plants comprising a truncated gene of the subject invention, and transgenic plants that produce these truncated proteins. This invention further provides methods of controlling plant pests, including rootworms, with these truncated proteins. The truncated Cry35 proteins of the subject invention are preferably used in combination with Cry34 proteins, which are known in the art. Various surprising advantages of the subject invention will be apparent in light of this disclosure.

REFERENCES:
patent: 5605793 (1997-02-01), Stemmer
patent: 6677148 (2004-01-01), Narva et al.
patent: WO 97/40162 (1997-10-01), None
patent: WO 00/24904 (2000-05-01), None
patent: WO 00/66742 (2000-11-01), None
patent: WO 01/14417 (2001-03-01), None
Moellenbeck et al (2001, Nature Biotechnol. 19:668-672).
Guo et al (2004, Proc. Natl. Acad. Sci. USA 101: 9205-9210).
Baum et al, 2004, Appl. Environ. Microbiol. 70:4889-4898.
Ellis et al (2002, Appl. Environ. Microbiol. 68:1137-1145).
Patterson et al, 1995, Anal. Chem. 67:3971-3978.
Clark et al (1990, J. Bacteriol. 172:6759-6763).
Chaurand et al (1998, J. Am. Soc. Mass. Spectrom. 10:91-103).
de Maagd et al, 2001, Trends Genet. 17: 193-199.
Altschul, S.F. et al. “Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs”Nucleic Acids Research, 1997, pp. 3389-3402, vol. 25, No. 17.
Ellis, R.T. “NovelBacillus thuringiensisBinary Insecticidal Crystal Proteins Active on WEstern Corn Rootworm,Diabrotica virgifera virgiferaLeConte”Applied and Environmental Microbiology, Mar. 2002, pp. 1137-1145, vol. 68, No. 3.
Herman, R.A. et al. “Binary Insecticidal Crystal Protein fromBacillus thuringiensis, Strain PS149B1: Effects of Individual Protein Components and Mixtures in Laboratory Bioassays”Journal of Economic Entomology, 2002, pp. 635-639, vol. 95, No. 3.
Hofte, H. and Whiteley, H.R. “Insecticidal Crystal Proteins ofBacillus thuringiensis” Microbiological Reviews, Jun. 1989, pp. 242-255, vol. 53, No. 2.
Karlin, S. and Altschul, S.F. “Applications and Statistics for Multiple High-Scoring Segments in Molecular Sequences”Proc. Natl. Acad., Jun. 1993, pp. 5873-5877, vol. 90.
Karlin, S. and Altschul, S.F. “Methods for Assessing the Statistical Significance of Molecular Sequence Features by Using General Scoring Schemes”Proc. Natl. Acad. Sci., Mar. 1990, pp. 2264-2268, vol. 87.
Crickmore et al.Bacillus thuringiensisToxin Nomenclature Official Website, Full Toxin List, Cry35 proteins (Aug. 2, 2005).
NCBI Accession No. AAG41672; Publication Date: Sep. 26, 2002.
NCBI Accession No. AAG50117; Publication Date: Mar. 4, 2002.
NCBI Accession No. AAG50342; Publication Date: Mar. 4, 2002.
NCBI Accession No. AAK64561; Publication Date: Jul. 29, 2004.
NCBI Accession No. AAK64563; Publication Date: Jul. 29, 2004.
NCBI Accession No. AAK64566; Publication Date: Jul. 29, 2004.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Truncated Cry35 proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Truncated Cry35 proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Truncated Cry35 proteins will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-2618731

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.