Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Algae – media therefor
Reexamination Certificate
2011-08-30
2011-08-30
Leavitt, Maria (Department: 1633)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Algae, media therefor
C435S257200, C435S946000, C800S278000
Reexamination Certificate
active
08008061
ABSTRACT:
Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalgaPhaeodaclylurn tricornutumcan be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human andChlorella kessleriglucose transporters facilitated the uptake of glucose byP. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
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Allnutt F. C. Thomas
Apt Kirk Emil
Kyle David J.
Lippmeier James Casy
Leavitt Maria
Martek Biosciences Corporation
Sterne Kessler Goldstein & Fox P.L.L.C.
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