Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2011-05-10
2011-05-10
Leavitt, Maria (Department: 1633)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C800S288000, C800S290000
Reexamination Certificate
active
07939710
ABSTRACT:
Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalgaPhaeodaclylurn tricornutumcan be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human andChlorella kessleriglucose transporters facilitated the uptake of glucose byP. tricornutum, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
REFERENCES:
patent: 4235043 (1980-11-01), Harasawa et al.
patent: 4857467 (1989-08-01), Sreekrishna et al.
patent: 5100792 (1992-03-01), Sanford et al.
patent: 5130242 (1992-07-01), Barclay
patent: 5244921 (1993-09-01), Kyle et al.
patent: 5374657 (1994-12-01), Kyle
patent: 5397591 (1995-03-01), Kyle et al.
patent: 5407957 (1995-04-01), Kyle et al.
patent: 5492938 (1996-02-01), Kyle et al.
patent: 5550156 (1996-08-01), Kyle
patent: 5567732 (1996-10-01), Kyle et al.
patent: 5658767 (1997-08-01), Kyle
patent: 5661017 (1997-08-01), Dunahay et al.
patent: 5711983 (1998-01-01), Kyle et al.
patent: 6027900 (2000-02-01), Allnutt et al.
patent: 2008/0138851 (2008-06-01), Apt et al.
patent: 0108580 (1984-05-01), None
patent: 0 235 112 (1987-09-01), None
patent: 3076583 (1991-04-01), None
patent: WO 93/05163 (1993-03-01), None
patent: WO 94/20627 (1994-09-01), None
patent: WO 96/31612 (1996-10-01), None
patent: 9739106 (1997-10-01), None
R. Lemoine et al., Identification of a pollen-specific sucrose transporter-like protein NtSUT3 from tobacco. FEBS Lett. 454 (1999), pp. 325-330.
Apt et al., Mol Gen Genet. Oct. 16, 1996;252(5):572-9.Stable nuclear transformation of the diatom Phaeodactylum tricornutum.
Asano et al., J Biol Chem. Dec. 25, 1991;266(36):24632-6. The role of N-glycosylation of GLUT1 for glucose transport activity.
Gladue (1991) “Heterotrophic Microalgae Production: Potential for Application to Aquaculture Feeds, Rotifer and Microalgae Culture Systems.” Proceedings of a U.S.—Asia Workshop, Honolulu, HI.
Gladue, R., “Heterotrophic Microalgae Production: Potential for Application to Aquaculture Feeds,”Rotifer and Microalgae Culture Systems, Proceedings of a U.S., Asia Workshop, The Oceanic Institute, (1991).
Carić, J., et al., “Dietary effects of different feeds on the biochemical composition of the rotifer (Brachionus plicatilisMüller),”Aquaculture, 110; 141-150 (1993).
Watanabe, T., “Importance of Docosahcxacnoic Acid in Marine Larval Fish,”J. World Aquacult Soc., 24; 152-161 (1993).
Apt, K.E., et al., “Commercial Developments in Microalgal Biotechnology,”J. Phycol., 35:215-226 (1999).
Chen, Feng, “High Cell Density Culture of Microalgae in Heterotrophic Growth,”TibTech, 14:421-426 (1996).
Dunahay, Terri G., “Genetic Transformation of the DiatomsCyclotella crypticaandNavicula saprophila,” J. Phycol., 31:1004-1012 (1995).
Fischer, Harald, et al., Targeting and Covalent Modification of Cell Wall and Membrane Proteins Heterologously Expressed in the DiatomCylindrotheca fusiformis(Bacillariophyceae),J. Phycol., 35:113-120 (1999).
Stevens, David R., “Genetic Engineering of Eukaryotic Algae: Progress and Prospects,”J. Phycol., 33:713-722 (1997).
Caspari, Thomas, et al., “Hexose/H+ Symporters in Lower and Higher Plants,”J. Exp. Biol., 196:483-491 (1994).
Amla, D.V., et al., “Metabolic Changes Associated with Cyanophage N-1 Infection of the CyanobacteriumNostoc Muscorum,” Arch. Microbiol., 148:321-327 (1987).
Beuf, Laurent, et al., “A Protein Involved-in Co-Ordiated Regulation of Inorganic Carbon and Glucose Metabolism in the Facultative Photoautotrophic CyanobacteriumSynechocystisPCC6803,”Plant Molecular Biology, 25:855-864 (1994).
Charng, Yee-yung, et al., “Structure-Function Relationships of Cyanobacterial ADP-Glucose Pyrophosphorylase,”The Journal of Biological Chemistry, 269(39):24107-24118 (1994).
Dunahay, Terri G., et al., “Genetic Engineering of Microalgae for Fuel Production,”Applied Biochemistry and Biotechnology, 34/35:331-339 (1992).
Hallmann, Armin, et al., “The Chlorella Hexose/H+ Symporter is a Useful Selectable Marker and Biochemical Reagent When Expressed inVolvox,” Proc. Natl. Acad. Sci. USA, 93:669-673 (1996).
Caceres, Odecio, “Some Features on the Growth of Microcystis Aeruginosa Kuetz, Emend, Elenkin in ASM- Medium,”Rev. Microbiol., Sao Paulo, 19(3):223-228 (1988).
Matsuoka, Makoto, et al., “Expression of Photosynthetic Genes from the C4Plant, Maize, in Tobacco,”Mol. Gen. Genet., 225:411-419 (1991).
Walmsley, A.R., et al., “Sugar Transporters from bacteria, Parasites and Mammals: Structure-Activity Relationships,”Trends Biochem. Sci., 23(12):476-481 (1998). Abstract Only.
Barrett, M.P., et al., “Trypanosome Glucose Transporters,”Mol. Biochem. Parasitol, 91(1):195-205 (1998). Abstract Only.
Mueckler, M., et al., “Sequence and Structure of a Human Glucose Transporter,”Science, 6:941-945 (1985).
Milbrandt, B., et al., “Glucose-Transport-Deficient Mutants of Schizosaccharomyces Pombe: Phenotype, Genetics and Use for Genetic Complementation,”Microbiology, 140:2617-2623 (1994). Abstract Only.
Henderson, P.J., “The Homologous Glucose Transport Proteins of Prokaryotes and Eukaryotes,”Res. Microbiol., 141(3):316-328 (1990). Abstract Only.
Kruckeberg, A.L., “The Hexose Transporter Family ofSaccharomyces cerevisiae,” Arch. Microbiol., 166(5):283-292 (1996). Abstract Only.
Bisson, L.F. et al., “Yeast Sugar Transporters,”Crit. Rev. Biochem. Mol. Biol., 28(4):259-308, (1993). Abstract Only.
Apt. K. E., et al., Stable Nuclear Transformation of the DiatomPhaeodactylum Tricornutum, Mol. Gen. Genet., 252:572-579 (1996).
Cossar, J.D., “Thioredoxin as a Modulator of Glucose-6-Phosphate Dehydrogenase in a N2-Fixing Cyanobacterium,”Journal of General Microbiology, 130:991-998 (1984).
Charng, Yee-yung, “Mutagenesis of an Amino Acid Residue in the Activator-Binding Site of Cyanobacterial ADP-Glucose Pyrophosphorylase Causes Alteration in Activator Specificity,”Archives of Biochemistry and Biophysics,318(2):476-480 (1995).
Stevens, D.R., et al., Development of a Dominant Selectable Marker for Nuclear Transpormation ofChlamydomonas reinhardtii, Journal of Experimental Botany, 46:37 (1995).
Broedel, Sheldon E., “Growth-Phase-Dependent Induction of 6-Phosphogluconate Dehydrogenase and Glucose 6-Phosphate Dehydrogenase in theCyanobacterium Synechococcussp. PCC7942,”Gene, 109:71-70 (1991).
Adhikary, S.P., “Utilization of Organic Substrates by Two Filamentous Cyanobacteria Under Various Growth Conditions,”Acta. Microbiologica Hundarica, 35:101-106 (1988).
Droop, M.R., “Heterotrophy of Carbon,” inAlgal Physiology and Biochemistry, Stewart, W.D.P., ed., University of California Press, Berkeley, CA USA, pp. 530-559 (1974).
Hellebust, J.A. and Lewin, J., “Heterotrophic Nutrition,” inThe Biology of Diatoms, Werner, D., ed. University of California Press, Berkeley, CA USA, pp. 169-197 (1977).
Hu, D., et al., “The C.reinhardtiiCF1with the mutation βT168S has
Allnutt F. C. Thomas
Apt Kirk Emil
Kyle David J.
Lippmeier James Casey
Leavitt Maria
Martek Biosciences Corporation
Sterne Kessler Goldstein & Fox P.L.L.C.
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