Triple helix purification and sequencing

Details Triple helix purification and sequencing Triple helix purification and sequencing

1992-07-16

1995-03-28

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 915, 536 231, 536 243, 536 2433, 935 6, 935 17, 935 78, C12Q 170, C07H 2104, C12P 1934

Patent

active

054016320

ABSTRACT:
Disclosed herein are methods, kits, and equipment for purifying single stranded circular DNA and then using the DNA for DNA sequencing purposes. Templates are provided with an insert having a hybridization region. An elongated oligonucleotide has two regions that are complementary to the insert and the oligo is bound to a magnetic anchor. The oligo hybridizes to the insert on two sides to form a stable triple helix complex. The anchor can then be used to drag the template out of solution using a magnet. The system can purify sequencing templates, and if desired the triple helix complex can be opened up to a double helix so that the oligonucleotide will act as a primer for further DNA synthesis.

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