Triple-helix forming oligonucleotides for targeted mutagenesis

Details Triple-helix forming oligonucleotides for targeted mutagenesis Triple-helix forming oligonucleotides for targeted mutagenesis

1995-06-07

1999-10-05

Myers, Carla J.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

536 231, 536 243, 536 253, 536 245, 435 6, A16K 4800, C07H 2104, C12Q 168

Patent

active

059624264

ABSTRACT:
A high affinity, triplex-forming oligonucleotide and methods for use thereof wherein an oligonucleotide is used to form a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the binding of the oligonucleotide stimulates mutagenesis within or adjacent to the target sequence using cellular DNA synthesis or repair mechanisms. The mutation activates, inactivates or alters the activity and function of the target molecule.

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