Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-06-14
1995-08-01
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 2, 436518, 436526, 436531, 436811, G01N 3353
Patent
active
054379873
ABSTRACT:
A method for separating nucleated fetal red blood cells and nucleated fetal cells and maternal granular sites from maternal blood is achieved by applying maternal blood to a triple gradient gel, isolating nucleated fetal cells from the gel and binding the isolated fetal cells to a solid support by means of an anti-i antibody bound to the solid support. The separated fetal cells can then be subjected to analysis for fetal sex or genetic disorders.
REFERENCES:
patent: 3852194 (1974-12-01), Zine, Jr.
patent: 4147628 (1979-04-01), Bennett et al.
patent: 4153739 (1979-05-01), Kessler
patent: 4190535 (1980-02-01), Luderer et al.
patent: 4255256 (1981-03-01), Ferrante et al.
patent: 4350593 (1982-09-01), Kessler
patent: 4675286 (1987-06-01), Calenoff
patent: 4751001 (1988-06-01), Saunders
patent: 4822745 (1989-04-01), Burns et al.
patent: 4925786 (1990-05-01), Uthemann
patent: 4927750 (1990-05-01), Dorn
Bhat et al, "One step separation of human fetal lymphocytes from nucleated redblood cells", in J. of Immunol. Methods v. 131 (1990) pp. 147-149.
Bhat et al, "One step enrichment of nucleated red bloods cells" in J. of Immunol. Methods, v. 158 (1993) pp. 277-280.
Okada et al, "Glycolipid antigens with Blood Group I and i specificities from Human Adult and Umbilical Cord Erythrocytes" in J of Immunology, v. 133, No. 2 (Aug. 1984) pp. 835-842.
Bianchi et al; Direct Hybridization to DNA from Small Numbers of Flow-Sorted Nucleated Newborn Cells, Cytometry 8: pp. 197-202 (1987); (Received for Publi Aug. 8, 1986, accepted Oct. 22, 1986.
Iverson et al; Detection and Isolation of Fetal Cells from Maternal Blood Using the Flourescence-Activated Cell Sorter (FACS), Prenatal Diagnosis, vol. 1, pp. 61-73 (1981).
Herzenberg et al; Fetal Cells in the Blood of Pregnant Women: Detection and Enrichment by Fluorescence-Activated Cell Sorting Proc. Natl. Acad. Sci USA, vol. 76, No. 3 pp. 1453-1455, Mar. 1979.
Bianchi et al; Isolation of Fetal DNA from Nucleated Erythrocytes in Maternal Blood Proc. Natl. Acad. Sci. USA, vol. 87 pp. 3279-3283, May 1990 Medical Sciences.
Corash et al; Separation of Erythrocytes According to Age on a Simplified Density Gradient J. Lab. Clin. Med, Jul. 1974, vol. 84 No. 1, pp. 147-151.
Bhat Neelima M.
Bieber Marcia
Teng Nelson N. H.
Spiegel Carol A.
The Board of Trustees of the Leland Stanford Junior University
LandOfFree
Triple gradient process with antibody panning to recover nucleat does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Triple gradient process with antibody panning to recover nucleat, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Triple gradient process with antibody panning to recover nucleat will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-733469