Triphenyl methane derivatives and method of quantitatively measu

Details Triphenyl methane derivatives and method of quantitatively measu Triphenyl methane derivatives and method of quantitatively measu

1984-12-19

1987-06-16

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving uric acid

435 11, 435 14, 435 25, 435 28, 436 71, 436135, 436904, C12Q 126, C12Q 128, C12Q 154, C12Q 160

Patent

active

046736352

ABSTRACT:
Disclosed herein is a novel compound which is excellent in solubility to water around a neutral range, stable in an aqueous solution state for a long period of time, and high in coloring sensitivity with a stable coloring development, and is represented by the general formula (I): ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which may be the same as or different from one another, represent lower alkyl groups, and X.sub.1 and X.sub.2 both represent --O(CH.sub.2).sub.n SO.sub.3 M in which M is a hydrogen atom, an alkali metal ion or NH.sub.4.sup.+, and n is an integer of 2-4, or either of X.sub.1 and X.sub.2 represents --O(CH.sub.2).sub.n SO.sub.3 M in which M and n are the same meanings as given above and the other represents a hydrogen atom. This reagent is hardly susceptible of the influence of the serum components such as hemoglobin, bilirubin and so on due to its color having absorption on a longer wavelength side. Disclosed is also a method of quantitatively measuring an oxidative substance, particularly, hydrogen peroxide, by using this color test reagent.

REFERENCES:
patent: 4066408 (1978-01-01), Jonsson et al.
M. Ikawa, Meth. Enzymology, 89, 145-146, 1982.

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