Trimerizing polypeptides

Details Trimerizing polypeptides Trimerizing polypeptides

2010-01-13

2011-12-27

Monshipouri, Maryam (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

Reexamination Certificate

active

08084230

ABSTRACT:
The present invention relates to a method of preparing a trimeric protein comprising culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a ZymoZipper (ZZ) domain and a heterologous protein. In one embodiment, the heterologous protein is a membrane protein, the portion of the heterologous protein that included in the fusion protein is the extracellular domain of that protein, and the resulting fusion protein is soluble. In another embodiment of the present invention, the ZZ domain is derived from the transmembrane (TM) subunit of a virus envelope protein or another heptad repeat containing gene of a virus genome. The method can be used to produced homo- and hetero-trimeric proteins. The present invention also encompasses DNA molecules, expression vectors, and host cells used in the present method and fusion proteins produced by the present method.

REFERENCES:
patent: 6190886 (2001-02-01), Hoppe et al.
patent: 6911205 (2005-06-01), Sodroski et al.
patent: 2004/033486 (2004-04-01), None
Amdt et al., “Comparison of In Vivo Selection and Rational Design of Heterodimeric Coiled Coils,”Structure, vol. 10, pp. 1235-1248, Sep. 2002.
Benit et al., “Identification, Phylogeny, and Evolution of Retroviral Elements Based on Their Envelope Genes,”Journal of Virology, vol. 75, No. 12, pp. 11709-11719, Dec. 2001.
Bullough et al., “Structure of influenza haemagglutinin at the pH of membrane fusion,”Nature, vol. 371, pp. 37-43, Sep. 1, 1994.
Cheynet et al., “Synthesis, Assembly, and Processing of the Env ERVWE1/Syncytin Human Endogenous Retroviral Envelope,”Journal of Virology, pp. 5585-5593, May 2005.
Fass et al., “Retrovirus envelope domain at 1.7 A resolution,”Nature Structural Biology, vol. 3, No. 5, pp. 465-469, May 5, 1996.
Fass and Kim, “Dissection of a retrovirus envelope protein reveals structural similarity to influenze hemagglutinin,”Current Biology, vol. 5, No. 12, pp. 1377-1383, 1995.
Harbury et al., “High-Resolution Protein Design with Backbone Freedom,”Science, vol. 282, pp. 1462-1467, Nov. 20, 1998.
McGrew et al., “Expression of Trimeric CD40 ligand inPichia pastorisis: use of a rapid method to detect high-level expressing transformants,”Gene: An International Journal on Genes and Genomes, vol. 187, No. 2, pp. 193-200, Mar. 18, 1997.
Muller et al., “Protein Fusions to Coiled-Coil Domains,”Methods in Enzymology, vol. 328, pp. 261-283, 2000.
Ogihara et al., “The crystal structure of the designed trimeric coiled coil coil-VaLd: Implications for engineering crystals and supramolecular assemblies,”Protein Science, vol. 6, pp. 80-88, 1997.
Thomas et al., “A trimeric alpha-helical, coiled coil peptide: association stoichiometry and interaction strength by analytical ultracentrifugation,”Eur Biophys J., vol. 25, pp. 405-410-, 1997.
Wagschal et al., “De Novo Design of a Model Peptide Sequence to Examine the Effects of Single amino Acid Substitutions in the Hydrophobic Core on both Stability and Oligomerization State of Coiled-coils,”J. Mol. Biol., vol. 285, pp. 785-803, 1999.
Chambers et al., “Heptad Repeat Sequences are Located Adjacent to Hydrophobic Regions in Several Types of Virus Fusion Glycoproteins,”Journal of General Virology, Society for General Microbiology, vol. 71, No. Part 12, pp. 3075-3080, Dec. 1990.
Delwart et al., “Retroviral Envelope Glycoproteins Contain a Leucine Zipper-Like Repeat,”Aids Research and Human Retroviruses, vol. 6, No. 6, pp. 703-706, Jun. 1990.

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