Trigonopsis transformant producing D-amino acid oxidase

Details Trigonopsis transformant producing D-amino acid oxidase Trigonopsis transformant producing D-amino acid oxidase

1993-07-23

1995-09-26

Walsh, Stephen G.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435 49, 435189, 435911, 536 232, C12N 115, C12N 1553, C12N 902, C12P 3506

Patent

active

054533740

ABSTRACT:
A Trigonopsis variabilis transformed with a recombinant DNA containing a D-amino acid oxidase gene capable of expressing in Trigonopsis variabilis is provided. A process for transforming Trigonopsis variabilis and a process for preparing 7-.beta.-(5-carboxy-5-oxopenetaneamide)cephalosporanic acid by using a transformant of Trigonopsis variabilis are also provided. The transformant of Trigonopsis variabilis shows high DAO activity and low activity of an esterase which interferes with the preparation of cephalosporin C. Accordingly, the Trigonopsis variabilis of the present invention enables one to produce cephalosporin C.
Moreover, the Trigonopsis variabilis of the present invention can be used for the preparation or cephalosporin C merely by treating the cells with toluene so that large scale use is practical.

REFERENCES:
patent: 3801458 (1974-04-01), Fildes et al.
patent: 5284754 (1994-02-01), Bayer et al.
Verdoes et al "Glucoamylase overexpression in Aspergillus niger . . . " Transgenic Res. 2:84-92.
Biotechnology Leters, vol. 14, No. 3 (Mar. 1992) pp. 195-200, Huber et al.
Bio/technology, vol. 9 (Feb. 1991) pp. 188-191, Isugai et al.
Isogai et al "Structure and Expression of cDNA for D-Amino Acid Oxidase . . . " J. Biochem. 108:1063-1069. (1990).

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