Triglycerides determination method

Details Triglycerides determination method Triglycerides determination method

1975-12-15

1977-08-30

Tanenholtz, Alvin E.

Textiles: fiber preparation

204 1T, 204195B, G01N 3114

Patent

active

040452972

ABSTRACT:
A method of determining triglycerides in biological fluids which comprises reacting a biological fluid sample with a lipolytic enzyme to liberate free glycerol, reacting said free glycerol with a coupled enzyme series in an oxygenated aqueous solution containing ATP, GK, .alpha.-GPDH, NAD and an electron transfer agent, and measuring the uptake of oxygen by the oxidation of the resulting NADH with an oxygen-sensing electrode.

REFERENCES:
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3759793 (1973-09-01), Stork et al.
patent: 3862009 (1975-01-01), Wahlefeld et al.
patent: 3898130 (1975-08-01), Komatsu
Greenbaum et al., "The Estimation of the Oxidized and Reduced Forms of the Nicotinamide Nucleotides," Biochem. J. 95 (1965), pp. 161-166.
Wieland, "Enzymatic Determination of Glycerol," Chemical Abstracts, vol. 52, (1958), No. 4732c.

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