Triglycerides assay and reagents therefor

Details Triglycerides assay and reagents therefor Triglycerides assay and reagents therefor

1977-12-07

1981-01-13

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 19, 435 26, C12Q 132, C12Q 144

Patent

active

042450411

ABSTRACT:
An improved triglyceride assay and reagent system which provides the introduction of iron into a reaction mixture in which glycerol from hydrolyzed triglycerides is coupled to a NAD/NADH indicator system, and measuring the resultant change of the oxidation state of the iron by use of an iron chelator, thereby improving the sensitivity and performance of the assay.

REFERENCES:
patent: 3331752 (1967-07-01), Struck et al.
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3915647 (1975-10-01), Wright
patent: 4038146 (1977-07-01), Nonaka et al.
patent: 4056442 (1977-11-01), Huang et al.
Bergmeyer, Methods of Enzymatic Analysis, vol. 1, Academic Press, Inc., New York, 1974, pp. 136-144.
Labouheur et al., Bull. Soc. Chim. Biol., 48, 747, (1966).
Boehringer Mannheim Catalog, No. 15558, p. 106, (1975).
Colowick et al., Methods in Enzymology, vol. 1, Academic Press, Inc., New York, 627 and 639, (1955).
Webster's Dictionary of the English Language, Unabridged "Encyclopedic Edition", 1131, (1977).

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