Trifunctional conjugates

Details Trifunctional conjugates Trifunctional conjugates

1995-03-22

1997-08-26

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

435 79, 435 792, 435 793, 435177, 435181, 435964, 436518, 436528, 436532, 436537, 436544, 436545, 436819, 530810, 530812, 530816, C12N 1100, C12N 1106, G01N 33547, G01N 33532

Patent

active

056610190

ABSTRACT:
A trifunctional conjugate is providing having three chemical moieties attached through a spacer moiety. At least two of the chemical moieties are relatively small molecules, usually less than about 7,000 Daltons in size. The spacer moiety is selected to impart certain steric properties to the conjugate. In one embodiment, the binding of a macromolecular specific binding partner to one of the chemical moieties sterically inhibits the binding of a different macromolecule to another chemical moieties. In another embodiment, the binding of a first chemical moiety to a macromolecule restricts the subsequent binding of a second tridentate member to a proximate location on the same macromolecule. The three chemical moieties are preferably a nitrophenylazido residue, a phenyl boronic acid residue, and a solid support or a label such as biotin. The spacer is preferably cysteine, lysine, glutamic acid, pyroglutamic acid, S-acetylmercaptosuccinic anhydride or .omega.-carbobenzoxylysine. The conjugate is useful in immunoassays and for targeted labeling of proteins.

REFERENCES:
patent: 3935074 (1976-01-01), Rubenstein et al.
patent: 4067774 (1978-01-01), Rubenstein et al.
patent: 4130462 (1978-12-01), Rubenstein et al.
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4228237 (1980-10-01), Hevey et al.
patent: 4233402 (1980-11-01), Maggio et al.
patent: 4238565 (1980-12-01), Horaby et al.
patent: 4243749 (1981-01-01), Sadeh et al.
patent: 4298685 (1981-11-01), Fairkh et al.
patent: 4506009 (1985-03-01), Lenhoff et al.
patent: 4550075 (1985-10-01), Bacquet et al.
patent: 4582789 (1986-04-01), Sheldon, III et al.
patent: 4604365 (1986-08-01), O'Neill et al.
patent: 4608336 (1986-08-01), Benovic et al.
patent: 4629690 (1986-12-01), Weng et al.
patent: 4663278 (1987-05-01), Diello et al.
patent: 4680338 (1987-07-01), Sundoro
patent: 4687732 (1987-08-01), Ward et al.
patent: 4687735 (1987-08-01), Diello et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4722906 (1988-02-01), Guire
patent: 4760142 (1988-07-01), Primes et al.
patent: 4791067 (1988-12-01), Sheiman et al.
patent: 4868104 (1989-09-01), Kurn et al.
patent: 5168057 (1992-12-01), Oh et al.
patent: 5196351 (1993-03-01), Harris et al.
Brigati, D., et al. Virology 126: 32-50 (1983).
Nardelli, et al., Journal Immuno, Methods, 120 (1989) 233-29.
Perelson (1980) Math. Biosci. 49 (1-2); 87-110.
Redeuilh, et al., Journal of Biol. Chem., 260(7) Apr. 1985, pp. 3996-4002.
Scott, et al., "Immunogenicity of Biotinylated Hapten-Aviden Complees", Molecular Immunology, 21, 1055-1060 (1984).
Sternberg, J.C., Chapter 6, Manual of Chemical Laboratory Immunology, 3rd Ed. (1986), pp. 33-37.
Stevenson, et al., "Tridentate Ligands Derived from Substitution in the Methyl Group of 8-Hydroxyquinaldine", Analytical Chemistry, 39, 1354-1358 (1967).
Wilchek, et al., Anal. Biochem., 171, 1-32 (1988).
Green, M.M., et al., "The Use of Bifunctional Biotinyl Compounds . . . ", Biochem J. 125:781-791 (1971).
Green, N.M., et al., "Electronic Microscopy of Complexes . . . ", J. Mol. Biol. 56:203:206 (1971).
Stevenson, R.L. and Freiser, H. "Tridentate Ligands derived from substitution . . . ", Chem. Abs. 67:9736, Abstract 103293K (1967).
Microbiology, 3d. Ed., "Immunology" pp. 292-295, 298-317, 324-355. Davis, B.D., et al, Eds. Harper & Row (1980).
Flygare et al., "Affinity Precipitation of Enzymes", Appl. Biochem. and Biotec., 7, 59-61 (1982).
Flygare et al., "Affinity Precipitation of Dehydrogenases", Anal. Biochem. 133, 409-416 (1983).
Gibbons et al., "Homogeneous Enzyme Immunoassay for Proteins Employing B-Galatosidase", Anal. Biochem., 102, 167-170 (1980).
Green et al., "The Use of Bifunctional Biotinyl Compounds to Determine the Arrangement of Subunits in Avidin", Biochemistry, 125, 781-791 (1971).
Guire et al., "Photochemical Coupling of Enzymes to Mammalian Cells", Pharm Res. Com., 9, (2), 131-141 (1977).
Kohno et al., "A Novel Enzyme Immunoassay of Anti-Insulin IgC in Guinea Pig Serum", Biochem. Biophys. Res. Comm., 147 (2), 644-649 (1987).
Larsson et al., "Affinity Precipitation of Enzymes", Elsevier/Notyh-Holland Biochemical Press, 98 (2), 330-333 (1979).
Lee et al., "Synthesis of the Bifunctional Dinucleotire AMP-ATP and its Application in General Ligand Affinity Chromatography", J. Solid Phase Biochem., 2(1), 31-39 (1977).
Litman et al., "Enzyme Channeling Immunoassay: A New Homogenous Enzyme Immunoassay Technique", Anal Biochem., 106, 223-229 (1980).
Morris et al., "Flavin Adenine Dinucleotide as a Label in Homogenous Colorimetric Immunoassays", Anal. Chem., 53(4), 658-665 (1981).
Ngo et al., "Enzyme Modulators as Tools for the Development of Homo-Geneous Enzyme Immunoassays", Elsevier/North-Holland Biomedical Press, 116(2), 285-288 (1980).
Oellerich, "Enzyme Immunoassay: A Review", J. Clin. Chem. Biochem., 22, 895-904 (1984).
Patel et al., "Chemiluminescence Energy Transfer: A New Technique Applicable to the Study of Ligand-Ligand Interactions in Living Systems", Anal. Biochem., 129, 162-169 (1983).
Simpson et al., "Chemiluminescent Labels in Immunoassay", in: Deluca et al., Bio-luminescence and Chemiluminescence, 673-679 (cademic Press, 1981).
Smith et al., "The Utility of Photo-Affinity Labels as `Mapping` Reagents", Biochem. J. 141, 51-56 (1974).
Sternberg, "A rate Nephelometer for Measureing Specific Proteins by Immunoprecipitin Reactions", Clin. Chem. 23(8), 1456-1464 (1977).
Stryer, "Fluorescence Eneergy Transfer as a Spectroscopic Ruler", Ann. Rev. Biochem., 47, 819-846 (1978).
Ullman et al., "Fluorescent Excitation Transfer Immunoassay", J. Biol. Chem., 251(14), 4172-4178 (1976).
Weeks et al., "Acridinium Esters as High Specific Activity Labels in Immunoassay", Clin. Chem., 29, (8), 1474-1479 (1983).
Whitehead et al., "Analytical Luminescence: Its Potential in the Clinical Laboratory", Clin Chem., 25(9), 1531-1545 (1979).
Product Publication: Microgenics' CEDIA Digoxin Assay Product Insert (Microgenics Corp., Concord, CA.), Mar. 1987.
Alkan S.S., et al., "Antigen Recognition And The Immune Response . . . ", J. Exp. Med. 135:1228-1246 (1972).
Tang, S.C., et al., "Chromium (III) asidoaquo complexes . . . ", Chem Abs., Section 78-Inorganic Chem.77: 147107p (1972).
Bush, M.E., et al., "Antigen Recognition and the Immune Response . . . ", J. Exp. Med. 136:1478-1483 (1972).
Woods, V., et al., "The Capacity of Bifunctional Antigens . . . ", Immunochemistry 12:379-382 (1975).

Affiliated with

Also associated with

Rating

Trifunctional conjugates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Trifunctional conjugates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Trifunctional conjugates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1987613