Trichoplusia ni cell line which supports replication of baculovi

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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435245, C12N 500, C12N 136

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053004353

ABSTRACT:
An insect cell line has been established and characterized, derived from embryonic tissue (BTI-TN-5B1-4, ATCC CRL 10859) of Trichoplusia ni (cabbage looper). The line is susceptible to various baculoviruses, including TnSNPV and AcMNPV, and may be used to replicate such viruses for use as insecticides or otherwise.

REFERENCES:
patent: 5024947 (1991-06-01), Inlow et al.
Rockford et al., In Vitro, vol. 20 No. 11 (Nov. 1984) pp. 823-825.
Granados et al., Virology, 152 (1986) pp. 472-476.
Jakoby et al., Methods in Enzymology, vol. LVIII, published 1979, pp. 450-466.
Hink, W. F. et al., Expression of Three Recombinant Proteins Using Baculoviurs Vectors in 23 Insect Cell Lines, biotechnol. Prog, 1991, 7, 9-14.
Wickham, T. J. et al., Screening of Insect Cell Lines for the Recombinant Proteins and Infectious Virus in the Baculovirus Expression System, Biotechnol. Prog. 1992, 8, 391-396.
Gibco Laboratories, Sf-900 Serum-Free Insect Cell Culture Medium with L-glutamine Catalog Number 350-0900, Oct. 1989, 4 pages.

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