Trichoderma reesei glucoamylase and homologs thereof

Details Trichoderma reesei glucoamylase and homologs thereof Trichoderma reesei glucoamylase and homologs thereof

2005-10-07

2008-08-19

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S069100, C435S254110, C435S254600, C435S320100, C435S484000, C435S252300, C536S023100, C536S023200

Reexamination Certificate

active

07413887

ABSTRACT:
The present invention is related to glucoamylases having at least 80% sequence identity to aTrichodermaglucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

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Chambergo et al., “Elucidation of the metabolic fate of glucose in the filamentous fungusTrichoderma reeseiusing espressed sequence tag (EST) analysis and cDNA microarrays,”J. of Biological Chemistry, Apr. 19, 2002, V. 277 :16, pp. 13983-13988.
Database EMBL Nov. 20, 2003, “TrEST-A0518 TrEST-AHypocrea jecorinacDNA clone Tr-A0518 5′ similar to glucan 1,4-alpha- glucosidase (EC 3.2.1.3) precuror—Neurospora crassa, m RNA sequence.” Retrieved from EBI accession No. EM—PRO:CF944444.
Database EMBL Nov. 20, 2003, “TrEST-A0916 TrEST-AHypocrea jecorinac DNA clone Tr-A0916 5′ similar to glucan 1,4-alpha-glucosidase (EC 3.2.1.3) precursor—Neurospora crassa, mRNA sequence.” Retrieved from EBI accession No. EM—PRO:CF943925.
Database EMBL Nov. 20, 2003, “TrEST-A2623 Trest-AHypocrea jecorinaCDNA clone Tr-A2623 5′ similar to glucan1,4-alpha-glucosidase (EC 3.2.1.3) precuror—Neurospora crassa, MRNA sequence.” Retrieved from EBI accession No. EM—PRO:CF944284.
Database EMBL Apr. 26, 2003 “tric021xg09T.reeseimycelial culture, Version Apr. 3Hypocrea jecorinacDNA clone tric021xg09. mRNA sequence.”retrived from EBI accession No. EM—PRO:CB900226.
Database EMBL Feb. 6, 2002, “TrEST-A4050 TrEST-AHypocrea jecorinacDNAclone Tr-A4050 5′ similar to glucoamylase, mRNA sequence.” Retrieved from EBI accession No. EM—PRO:BM076396.
Fagerstrom et al., “Characterization, subsite mapping and partial bamino acid sequence of glucoamylase from the filamentous fungusTrichoderma reesei.” Biotechnology and Applied Biochemistry Res. Lab., Alko Ltd.,V.21 :2, 1995, pp. 223-231.
Fagerstrom et al., “Purification and specificity of recombinantHormoconis resinaeglucoamylase P and endogenous glucoamylase fromTrichoderma reesei,” Enzyme and Microbial Technology,V.16 :1, 1994, pp. 36-42.
Stone et al., “Cloning and Sequence Analysis of the Glucoamylase Gene ofNeurospora Crassa”, Current Genetics,V.24:3, 1993, pp. 205-211.
Takashima et al., “Molecular cloning and expression of the novel fungal beta-glucosidase fromH.griseaandT.reesei,” J.of Biochemistry, Japanese Biochemical Society,V.125 :4, Jan. 1999, pp. 728-736.
*Barnett et al., “Cloning and amplification of the gene encoding an extracellular beta-glucosidase fromTrichoderma reesei: Evidence for improved rates of saccharification of cellulosic substrates,”Bio/Technology, V.9 :9, Jun. 1991, pp. 562-567.
*Chambergo et al., “Elucidation of the metabolic fate of glucose in the filamentous fungusTrichoderma reeseiusing espressed sequence tag (EST) analysis and cDNA microarrays,”J. of Biological Chemistry,Apr. 19, 2002, V. 277 :16, pp. 13983-13988.
*Database EMBL Nov. 20, 2003, “TrEST-A0518 TrEST-AHypocrea jecorinacDNA clone Tr-A0518 5′ similar to glucan 1,4-alpha-glucosidase (EC 3.2.1.3) precuror—Neurospora crassa, m RNA sequence.” Retrieved from EBI accession No. EM—PRO:CF944444.
*Database EMBL Nov. 20, 2003, “TrEST-A0916 TrEST-AHypocrea jecorinacDNA clone Tr-A0916 5′ similar to glucan 1,4-alpha-glucosidase (EC 3.2.1.3) precursor—Neurospora crassa, mRNA sequence.” Retrieved from EBI accession No. EM—PRO:CF943925.
*Database EMBL Nov. 20, 2003, “TrEST-A2623 Trest-AHypocrea jecorinaCDNA clone Tr-A2623 5′ similar to glucan1,4-alpha-glucosidase (EC 3.2.1.3) precuror—Neurospora crassa, MRNA sequence.” Retrieved from EBI accession No. EM—PRO:CF944284.
*Database EMBL Apr. 26, 2003 “tric021xg09T.reeseimycelial culture, Version Apr. 3Hypocrea jecorinacDNA clone tric021xg09, mRNA sequence.”retrived from EBI accession No. EM—PRO:CB900226.
*Database EMBL Feb. 6, 2002, “TrEST-A4050 TrEST-AHypocrea jecorinacDNAclone Tr-A4050 5′ similar to glucoamylase, mRNA sequence.” Retrieved from EBI accession No. EM—PRO:BM076396.
*Fagerstrom et al., “Characterization, subsite mapping and partial bamino acid sequence of glucoamylase from the filamentous fungusTrichoderma reesei.” Biotechnology and Applied Biochemistry Res. Lab., Alko Ltd., V.21 :2, 1995, pp. 223-231.
*Fagerstrom et al., “Purification and specificity of recombinantHormoconis resinaeglucoamylase P and endogenous glucoamylase fromTrichoderma reesei,” Enzyme and Microbial Technology, V.16 :1, 1994, pp. 36-42.
*Stone et al., “Cloning and Sequence Analysis of the Glucoamylase Gene ofNeurospora crassa”, Current Genetics, V.24:3, 1993, pp. 205-211.
*Takashima et al., “Molecular cloning and expression of the novel fungal beta-glucosidase fromH.griseaandT.reesei,” J.of Biochemistry, Japanese Biochemical Society, V.125 :4, Jan. 1999, pp. 728-736.
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