Trehalostatin and process for the preparation thereof

Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Biocides; animal or insect repellents or attractants

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Details

424 934, 435100, 435132, 435155, 435169, 4352521, 435822, 435911, 536 187, A01N 6300, C12P 700, C12N 120, C07H 504

Patent

active

053728165

ABSTRACT:
This invention relates to the substance Trehalostatin which is a white powder soluble in water but hardly or only slightly soluble in hexane, benzene, ethers and petroleum ether, shows no absorption maxima at 220 nm or above in its ultraviolet visible light absorption spectrum, is positive in Rydon-Smith reaction and negative in nynhydrin reaction, 3,6-dinitrophthalic acid reaction and Elson-Morgan reaction, and has an RF value of 0.37 in Merck Kieselgal 50 F.sub.254 thin-layer chromatography using 3:1:2 mixture on n-butanol, acetic acid and water as a developing solvent, Rt of 11.0 minutes in YMC PA03 (0.7 .times.27 cm) high performance liquid chromatography using 65% v/v acetonitrile (in H.sub.2 O) as a solvent at a flow rate of 1.0 ml/min, a molecular weight of 366, [alpha].sub.D of +115.degree., and specific NMR spectrum.
Also disclosed is a process for preparing trehalostatin and a specific strain of Amycolatopsis trehalostatica which has FERM accession number BP-2784.
In addition the trehalostatin compound shows an inhibitory effect against trehalase in insects, especially Aldrichina grahami, even at a very low concentration and is therefore useful as an insecticide.

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