Trehalose phosphorylase its preparation and uses

Details Trehalose phosphorylase its preparation and uses Trehalose phosphorylase its preparation and uses

1997-11-07

1998-12-01

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 691, 4352523, 4353201, 530300, 530350, 536 232, C12N 910, C12P 2106, C07K 100, C07H 2104

Patent

active

058437487

ABSTRACT:
A thermostable trehalose phosphorylase which is obtainable from microorganisms of the genus Thermoanaerobium and which hydrolyzes trehalose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid. The trehalose phosphorylase can be also prepared by recombinant DNA technology. When the enzyme is allowed to contact with .beta.-D-glucose-1-phosphoric acid as a saccharide donor in the presence of other saccharides, glucosyl-transferred saccharides including glucosyl-D-galactoside, which are conventionally known but scarcely obtainable, can be produced on an industrial-scale and in a relatively-low cost.

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Kizawa, Hideki et al., "Purification and characterization of trehalose phosphorylase form micrococcus varians.", Biosci. Biotech. Biochem., vol. 59, No. 10, pp. 1908-1912 (1995).
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