Trehalose phosphorylase its preparation and uses

Details Trehalose phosphorylase its preparation and uses Trehalose phosphorylase its preparation and uses

1998-06-24

1999-03-02

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 72, 435 95, 435 97, 435 691, 4352523, 4353201, 530300, 530350, 536 232, C12P 1944, C12P 2106, C12N 910, C07K 100

Patent

active

058769757

ABSTRACT:
A thermostable trehalose phosphorylase which is obtainable from microorganisms of the genus Thermoanaerobium and which hydrolyzes trehalose in the presence of an inorganic phosphoric acid to form D-glucose and .beta.-D-glucose-1-phosphoric acid. The trehalose phosphorylase can be also prepared by recombinant DNA technology. When the enzyme is allowed to contact with .beta.-D-glucose-1-phosphoric acid as a saccharide donor in the presence of other saccharides, glucosyl-transferred saccharides including glucosyl-D-galactoside, which are conventionally known but scarcely obtainable, can be produced on an industrial-scale and in a relatively-low cost.

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Kizawa et al, "Purification and Characterization of Trehalose Phosphorylase from Micrococcus varians", Biosci. Biotech. Biochem. 59(10):1908-1912 (1995).
Murao et al, "Enzymatic Synthesis of Trehalose from Maltose", Agric. Biol. Chem. 49(7):2113-2118 (1985).
Marechal et al, "Metabolism of Trehalose in Euglena gracilis", J. Biol. Chem.247(10):3223-3228 (1972).
Sambrook et al, Molecular Cloning: A Laboratory Manual, 2nd. Ed., Cold Spring Harbor Laboratory Press (Cold Spring Harbor, NY, 1989).
Yoshida et al, "Production and Application of Maltose Phosphorylase and Trehalose Phosphorylase by a Strain of Plesiomonas", Oyo Tashitsu Kagaku 42(1):19-25 (1995).

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